鸡促卵泡素及其受体基因在多个非繁殖组织中的表达研究  

The Expression of Follicle Stimulating Hormone and Its Receptor Genes in Certain Non-reproductive Tissues of Chicken

作　　者：崔焕先[1] 赵桂苹[1] 刘冉冉[1] 郑麦青[1] 陈继兰[1] 文杰[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《畜牧兽医学报》2011年第11期1519-1525,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：现代农业产业技术体系建设专项资金(nycytx-42)

摘　　要：本研究利用PCR和免疫组化等方法,对FSHR和FSH基因在鸡多个非繁殖系统的组织器官中的mRNA和蛋白水平的表达以及相关性进行了分析。结果显示:在心脏、肝脏、肾脏、肺和十二指肠中FSHR mRNA和蛋白以及FSH蛋白水平具有相同的表达趋势,表达丰度从大到小依次为肝脏、心脏、肾脏、肺和十二指肠。而在脾脏、胃、腿肌和胸肌中未检测到FSHR mRNA和蛋白以及FSH蛋白水平的表达;在检测的9个组织中,均无FSHmRNA的表达。相关性分析结果表明,在肝脏、心脏、肾脏、肺和十二指肠中各组织器官质量与组织中的FSH蛋白水平具有显著或极显著的正相关(P<0.05或P<0.01);各组织中的FSHR mRNA和蛋白水平均与FSH蛋白水平存在显著或极显著正相关(P<0.05或P<0.01)。本研究初步证明,机体其他部位表达分泌的促卵泡素,在多个非繁殖组织器官(肝脏、心脏、肾脏、肺和十二指肠)中通过调控促卵泡素受体基因的表达来影响北京油鸡不同组织的发育。The mRNA and protein expressions of FSH and FSHR were detected in some non-reproductive tissues of Beijing-you chickens by PCR and immunohistochemistry in this study.Subsequently,their correlation analysis also was performed.The results revealed that all of the FSHR mRNA,FSHR and FSH protein levels in five detected tissues（liver,heart,kidney,lung and duodenum） had the same distribution trend,and their expression level from high to low were the liver,heart,kidney,lung and duodenum.But FSHR mRNA,FSHR and FSH protein were not expressed in the spleen,stomach,breast muscle and thigh muscle.And FSH mRNA was not expressed in nine tissues.There was the significant positive correlationship（P0.05 or P0.01） between the tissue weight and FSH level in liver,heart,kidney,lung and duodenum,and there were also the significant positive correlations（P0.05 or P0.01） between the level of FSHR mRNA or protein and the FSH level in different tissues.In conclusion,it had been preliminarily revealed that FSH,coming from blood circulation,might induce the expression of the FSHR gene to promote the tissues development of the heart,liver,lung,kidney and duodenum in chicken.

关 键 词：促卵泡素受体基因 表达 受体 促卵泡素 非繁殖组织 北京油鸡 

分 类 号：S831.2[农业科学—畜牧学] Q786[农业科学—畜牧兽医]