Toll样受体2基因单核苷酸多态性与肝细胞癌易感性的关系  

Association between Toll-like receptor 2 single nucleotide polymorphisms and susceptivity of hepatocellular carcinoma

作　　者：赵健全[1] 施敏敏[1] 谢俊杰[1] 金佳斌[1] 沈柏用[1] 邓侠兴[1] 詹茜[1] 彭承宏[1] 陈皓[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院外科上海消化外科研究所,上海200025

出　　处：《外科理论与实践》2011年第4期370-373,共4页Journal of Surgery Concepts & Practice

基　　金：国家自然科学基金(81070356);上海市科委基金(09411952200)

摘　　要：目的：研究Toll样受体2（TLR2）基因单核苷酸多态性（SNP）与肝癌易感性之间的关系。方法：采用病例组与对照组研究,收集211例肝细胞癌（肝癌组）和232例非肝细胞癌（对照组）外周血标本,对TLR2的5个SNP采用多重单碱基延伸SNP分型技术进行基因分型。采用卡方检验比较基因型在病例组与对照组之间分布的差异,采用非条件logistic回归分析多态基因型与肝细胞癌发生危险度的关系。结果：rs3804099、rs3804100基因型在肝癌组与对照组中的分布具有显著性差异（P〈0.05）。rs3804099和rs3804100带CT突变杂合子与带TT的野生型纯合子比较,发生HCC的危险性显著降低;经年龄、性别校正后OR值分别为0.493（95%CI：0.331~0.736）和0.509（95%CI：0.342~0.759）（P〈0.05）。单倍体TT可显著降低HCC发生的危险（OR 0.524,95%CI：0.394~0.697,P〈0.01）;相反,单倍体CC可增加肝细胞癌的发生（OR 2.743,95%CI：1.915~3.930,P〈0.01）。结论：TLR2基因SNP与肝细胞癌的发生显著相关。Objective To identify the role of TLR2 single nucleotide polymorphisms and susceptivity of hepatocellular carcinoma.Methods A systematic genetic analysis of sequence variants of TLR2 by evaluating five single nucleotide polymorphisms was performed from 211 hepatocellular carcinoma cases and 232 controls.Genomic DNA was extracted from peripheral blood leukocytes and genotyping was undertaken by using the SNaPshot method.The distribution differences of polymorphisms and related risk between the hepatocellular carcinoma group and the control group were analyzed by the Chi-square test.The relationship of polymorphism genotype with the risk of hepatocellular carcinoma was analyzed by logistic regression.Results Genetic variants of rs3804099、rs3804100 of TLR2 were associated significantly with hepatocellular carcinoma susceptivity（P0.05）.Individuals carrying the heterozygous genotypes of rs3804099 and rs3804100 had decreased risk of aequiring hepatocellular carcinoma（OR adjusted by sex and age 0.493,95%CI： 0.331-0.736;OR adjusted by sex and age 0.509,95%CI： 0.342-0.759）（P0.05）.The risk of acquiring hepatocellular carcinoma in individuals with haplotype TT decreased significantly（OR adjusted by sex and age 0.524,95% CI： 0.394-0.697）（P0.01）;inversely,the risk in individuals with haplotype CC increased（OR adjusted by sex and age 2.743,95% CI： 1.915-3.930）（P0.01）.Conclusions The genetic variants of TLR2 may be associated with the risk of occurrence of hepatocellular carcinoma.

关 键 词：单核苷酸多态性 肝细胞癌 TOLL样受体2 

分 类 号：R735.7[医药卫生—肿瘤]