再生障碍性贫血患者Th及NKT细胞IFN-γ和IL-4的表达及意义  

Expression and significance of IFN-γ and IL-4 in Th and NKT cells of patients with aplastic anemia

作　　者：童勇[1] 马莉[1] 曾小菁[2] 

机构地区：[1]贵阳医学院附属医院中心实验室,贵阳550004 [2]贵阳医学院附属医院血液内科,贵阳550004

出　　处：《中国免疫学杂志》2011年第9期840-843,共4页Chinese Journal of Immunology

基　　金：贵州省卫生厅资助项目(G2003-6)

摘　　要：目的:探讨Th及NKT细胞内细胞因子(Intracellular cytokine,ICK)在再生障碍性贫血(Aplastic anemia,AA)发病中所起的作用。方法:流式细胞术(FCM)检测Th细胞表面CD3+CD4+分子、NKT细胞表面CD3+CD16+56+分子,细胞内染色技术检测Th、NKT细胞内IFN-γ和IL-4水平。结果:AA患者外周血Th细胞减少(P<0.01),细胞内CD3+CD4+IFN-γ+、CD3+CD4+IL-4+均增高,Th1/Th2增高(P<0.01);NKT细胞增多(P<0.05),NKT细胞内IL-4+水平升高(P<0.05),IFN-γ+水平无统计学差异。结论:AA的发病与T淋巴细胞异常密切相关,是一种Th1型反应。NKT细胞可代偿调节AA患者IFN-γ和IL-4比例。Objective：To explore the role of intracellular IFN-γ and IL-4 of Th and NKT cells in the pathogenesis of aplastic anemia.Methods：Lymphocyte surface CD molecules were detected by FCM.Intracellular cytokines were measured through intracellular staining.Results：Th lymphocytes in peripheral blood of AA patients decreased.The level of intracellular CD3＋CD4＋IFN-γ＋ and CD3＋CD4＋IL4＋ increased,Th1/Th2 ratio was significant higher（P0.01）.NKT cells increased and its level of IL-4＋ was obviously higher（P0.05）.Conclusion：The amount and function of T lymphocytes are closely related to the pathogenesis of aplastic anemia patients.Aplastic anemia is the Th1-type bias.NKT cells vicariously regulate IFN-γ and IL-4 in patients with AA.

关 键 词：细胞内细胞因子 再生障碍性贫血 流式细胞术 

分 类 号：R733.7[医药卫生—肿瘤] R392.1[医药卫生—临床医学]