联合免疫疗法治疗小鼠肝癌移植瘤模型的研究  

Study on the therapeutic efficacy of united immunotherapy to mouse hepatoma model

作　　者：傅庆国[1] 朱林波[2] 李晔 应可明[2] 

机构地区：[1]宁波市第二医院肿瘤外科,宁波315010 [2]浙江省宁波大学医学院,宁波315211 [3]福建仙芝楼生物科技有限公司,福州350002

出　　处：《中国免疫学杂志》2011年第11期983-987,共5页Chinese Journal of Immunology

基　　金：浙江省卫生厅资助项目(No.2006B117)

摘　　要：目的:探讨基础免疫调节、非特异性免疫治疗和特异性免疫治疗联合运用对小鼠肝癌移植瘤的治疗作用和机理。方法:细胞培养技术培育小鼠的肝癌细胞,高效液相色谱和亲和层析技术分离肿瘤HSP70、SDS-PAGE电泳和Western blot技术进行HSP70的检测,以清洁级小鼠进行治疗实验,用ELISA讲行细胞因子的分析。结果:治疗组荷瘤鼠移植瘤重量、体积均显著减小,肿瘤转移率显著降低,生存时间明显延长,与对照组相比,差别有极显著性(P<0.05或P<0.01),其中三种药物联合治疗组荷瘤鼠肿瘤均消失,无转移,均获得长期生存(>90天);治疗组血清中IFNγ-、CXCL-9、CXCL-10水平较对照组均有升高,与对照组差异显著(P<0.05或P<0.01),以三种药物联合治疗组升高尤为显著。结论:联合免疫疗法可以改善肿瘤微环境,基础免疫调节对特异性和非特异性免疫治疗有促进和增强作用,研究证明联合免疫疗法对小鼠肝癌移植瘤具良好的治疗作用,该疗法对改善免疫治疗的临床应用策略有重要参考意义。Objective：To investigate the combined-therapeutic efficacy and mechanism of basic immunomodulation,non-specific immunotherapy and specific immunotherapy to tumor-bearing mice.Methods：Liver cancer cell was cultured with cell culture technique;tumor HSP70 was separated by high performance liquid chromatography and affinity chromatography technology;HSP70 was detected and analyzed by SPS-PAGE and Western blot technology;experiment of treatment were carricd out with virus free animals;cytokines and chemokines were analyzed by ELISA technique.Results：Average tumor weight of treated groups was significantly lightener and average tumor volume of treated groups was significantly smaller than control group（P0.05,P0.01）,the metastasis rate of treated groups was much lower than control group,the life span of treated groups was significantly longer comparing with control group（P0.01）,of all,in Co-use of Ganoderma spore,CpG-ODN and HSP70-PCs,all mice achieved long term survival without tumor.The IFN-γ,CXCL-9 and CXCL-10 level in serum of treated groups were much promoted contrasted with control group（P0.05,P0.01）,the level of Co-use of Ganoderma spore,CpG-ODN and HSP70-PCs was significantly elevated.Conclusion：Co-use of basic immunomodulation,non-specific immunotherapy and specific immunotherapy improved tumor microenvironment.Basic immunomodulation enhanced and promoted non-specific and specific immunotherapy.This study showed Co-use of basic immunomodulation,non-specific immunotherapy and specific immunotherapy had noticeable therapeutic effect on mouse hepatoma model,which offered significant information for the improvement of immunotherapy in clinical use.

关 键 词：HSP70-PCs 肿瘤 免疫治疗 

分 类 号：R730.51[医药卫生—肿瘤]