肿瘤干细胞标记CD44和CD133在鼻咽癌细胞株中的表达检测  

Expression of tumor stem cell marker CD44 and CD133 in human nasopharyngeal carcinoma cell line

作　　者：苏进[1] 黄乔[1] 许新华[1] 鲁明骞[1] 易芳[1] 

机构地区：[1]三峡大学第一临床医学院湖北省宜昌市中心人民医院肿瘤科,443002

出　　处：《广东医学》2011年第18期2367-2369,共3页Guangdong Medical Journal

基　　金：湖北省卫生厅科研项目(编号:JX4B52);湖北省宜昌市科技研究与开发项目(编号:A11301-04);湖北省宜昌市中心人民医院科研发展基金资助项目(编号:KFJ2008005)

摘　　要：目的探索肿瘤干细胞标记物CD44和CD133在鼻咽癌(NPC)中的表达量及其有效分选方式。方法常规培养SUNE-1 5-8F细胞,采用免疫荧光技术及流式细胞学技术检测SUNE-1 5-8F细胞中CD44、CD133的表达,并用流式细胞仪分选CD44+、CD44+CD133+细胞。结果激光共聚焦镜下鼻咽癌SUNE-1 5-8F细胞株细胞膜上可以观察到CD44分布;流式细胞学技术检测SUNE-1 5-8F中CD44+其表达率为42.4%～52.5%,经流式细胞仪分选得到的CD44+和CD44-细胞,其纯度分别达98.3%和97.9%。流式细胞仪检测到CD44+CD133+细胞(P2)在SUNE-1 5-8F细胞株中的表达约为3.1%。结论 SUNE-1 5-8F细胞株中肿瘤干细胞标记含量较高,适合鼻咽癌干细胞的研究,且流式细胞学技术不失为一种有效的分选细胞手段。Objective To evaluate the expression of the tumor stem cell（TSC） marker CD44 and CD133 in hu- man nasopharyngeal carcinoma （NPC） SUNE -15 -8F cell line. Methods Immunocytoehemistry and flow cytometry were used to assess the expression of CD44 and CD133 in SUNE - 15 - 8F cells. Fluorescence - activated cell sorting （FACS） was applied for purification of CD44＋ cells. Results Immunofluorescence analysis revealed that CD44 was expressed on some of SUNE - 1 5 - 8F ceils. CD44 positive ceils accounted for 42. 4% - 52. 5% of the total ceils. The purities of CD44＋ and CD44 - cells after sorting were 98.3% and 97.9% , respectively. Conclusion SUNE - 15 - 8F cell line with high expression of TSC marker is suitable for study of NPC TSC. FACS is effective in cell sorting.

关 键 词：鼻咽肿瘤 肿瘤干细胞 CD44 CD133 肿瘤干细胞标记 

分 类 号：R734.2[医药卫生—肿瘤]