热休克诱导虹鳟三倍体的最佳条件  

The best condition to induce triploid rainbow trout(Oncorhynchus mykiss) by heat shock

作　　者：张艳萍[1,2] 王太[1,2] 俞小牧[3] 娄忠玉 焦文龙 

机构地区：[1]甘肃省水产科学研究所甘肃省冷水性鱼类种质资源与遗传育种重点实验室,甘肃兰州730030 [2]甘肃农业大学动物科技学院,甘肃兰州730070 [3]中国科学院水生生物研究所,湖北武汉430072

出　　处：《西北师范大学学报（自然科学版）》2011年第6期69-74,共6页Journal of Northwest Normal University(Natural Science)

基　　金：甘肃省科技支撑计划项目(097JKCA080);甘肃省重点实验室建设计划(1009RTSA006)

摘　　要：以4龄道氏虹鳟雌鱼为母本,2龄道氏虹鳟雄鱼为父本,采用热休克法研究阻止第二极体排放诱导虹鳟三倍体最佳诱导条件,按正交实验方案组合诱导参数.结果表明:采用三个不同温度(26.5,28,30℃)进行诱导处理,均成功产生虹鳟三倍体.随着诱导温度的升高、处理持续时间的延长,虹鳟受精卵2h内死亡率逐渐上升;在30℃处理持续时间超过10min的条件下,虹鳟受精卵全部死亡或停止发育,认为30℃临近虹鳟发眼卵的致死温度;在26.5℃下,授精后15min起始处理10min,诱导效果最佳,发眼率为79.32%,诱导率达到83.18%,且诱导率与处理持续时间密切相关,研究结果可为规模化生产虹鳟三倍体提供参考依据.Heat shock treatment is utilized to deal with eggs of rainbow trout forming triploid,which by the retention of the second polar body of fertilized eggs.Based on eyed rate and triploidy rate for the analysis of variance comparison,three factors（treatment temperature,treatment processing time,treatment processing duration）are selected to investigate.The result shows that using heat shock treatment induced triploid rainbow trout and three temperatures are all successfully induced the triploid rainbow trout.As the shock temperature,the dead rate of fertilized eggs has increased within 2 h.In the 30 ℃ and treatment time lasts longer than 10min conditions rainbow trout eggs all died or stop the growth.So 30 ℃ is very close to the lethal temperature rainbow trout eggs.In other conditions,as the extension of treatment duration,the death rate of fertilized eggs has increased within 2 h.An effective procedure for inducing triploid is putting the fertilized egg into the 26.5 ℃ after 15min insemination to heat shock 10 min.Under that situation,about 83.18% triploidy embryos is obtained.It leads to conclusions that the most important factor for triploidy induction is duration time,the second is temperature and the third is initiative time of shock.

关 键 词：虹鳟 三倍体 热休克 倍性检测 

分 类 号：Q953.5[生物学—动物学]