SKI-Ⅱ对胃癌SGC7901细胞血管内皮生长因子表达的影响  

Effects of SKI-Ⅱon the expression of vascular endothelial growth factor in SGC7901 gastric cancer cells in vitro

作　　者：周红连[1] 费素娟[1] 朱祖安[1] 孙旻[1] 张秋月[1] 

机构地区：[1]徐州医学院附属医院消化内科,江苏徐州221000

出　　处：《广东医学》2011年第17期2229-2231,共3页Guangdong Medical Journal

摘　　要：目的观察SKI-Ⅱ对胃癌SGC7901细胞内血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响,并对其机制进行初步研究。方法常规培养胃癌SGC7901细胞,给予不同浓度SKI-Ⅱ及顺铂(DDP)干预,MTT法测定各组胃癌SGC7901细胞增殖情况,免疫组化和Western blot检测药物对SphK1、VEGF表达的影响。结果 MTT检测显示SGC7901细胞与不同浓度的SKI-Ⅱ共同培养,细胞的生长受到不同程度的抑制,其作用具有剂量依赖性,并随时间的延长逐渐增强,免疫组化及Western blot检测显示SKI-Ⅱ可显著下调肿瘤细胞SphK1和VEGF蛋白的表达,单用DDP对SphK1、VEGF无作用。结论 SKI-Ⅱ可以通过下调VEGF和SphK1蛋白表达而有效抑制胃癌SGC7901细胞增殖。Objective To investigate the effects and mechanism of SKⅠ-Ⅱ on the expression of vascular endo- thelial growth factor （VEGF） in gastric cancer SGC -7901 cells. Methods SGC7901 gastric cancer cells in vitro were treated with different concentrations of SKⅠ-Ⅱ or DDP. Cell proliferation was analyzed by MTT assay. And SphK1 and VEGF protein expression was assessed by immunohistochemistry and western blot. Results SGC7901 cell proliferation was inhibited by SKⅠ-Ⅱ in dose - dependent and time - dependent manners. The expression of SphK1 and VEGF in SGC7901 cells was reduced by SKⅠ-Ⅱ , but not by DDP. Conclusion SKⅠ-Ⅱ down - regulates SphK1 and VEGF proteins, thus inhibits the proliferation of SGC7901 cells.

关 键 词：SKI-Ⅱ 血管内皮生长因子 SphK1 SGC7901细胞 

分 类 号：R735.2[医药卫生—肿瘤]