硼替佐米和3-甲基腺嘌呤对人乳腺癌细胞株MCF-7的抑瘤效应  

Inhibition of Bortezomib and 3-Methyladenine on Cell Proliferation in Human MCF-7 Breast Cancer Cells

作　　者：姚峰[1] 王冠楠[1] 魏文[1] 涂毅[1] 童鹤翔[1] 孙圣荣[1] 

机构地区：[1]武汉大学人民医院乳腺甲状腺外科,武汉430060

出　　处：《微循环学杂志》2011年第4期7-9,12,I0002,共5页Chinese Journal of Microcirculation

摘　　要：目的:研究蛋白酶体活性抑制剂硼替佐米(Bortezomib)对人乳腺癌细胞株MCF-7的自噬诱导和增殖抑制作用,以及自噬抑制剂3-甲基腺嘌呤(3-MA)与Bortezomib联合应用对MCF-7细胞自噬及增殖的影响。方法:采用MTT法检测细胞增殖,蛋白质印迹法检测蛋白酶体活性及自噬相关蛋白LC3的表达。结果:Bortezomib能明显抑制MCF-7细胞增殖并诱导其自噬,3-MA与Bortezomib联合应用,可逆转Bortezomib诱导的细胞自噬,并增强Bortezomib对MCF-7细胞增殖的抑制(P<0.05)。结论:联合应用Bortezomib和3-MA可能对乳腺癌细胞自噬和增殖具有协同抑制作用。Objective：To investigate whether inhibition of the proteasome（proteasome inhibitor Bortezomib）can induce autophagy and the effect of combination inhibition of both proteasome and autophagy（autophagy inhibitor 3-Methyladenine,3-MA）on the human breast cancer MCF-7 cells.Method：Cell viability was measured by MTT assay.The expression of proteasome and autophagy related proteins was determined by Western blot.Results：MCF-7 cells proliferation was inhibited and autophagy was activated by Bortezomib.However,when MCF-7 cells were co-treated with Bortezomib and 3-MA,3-MA blocked the increase of LC3-II protein expression induced by Bortezomib and it could enhance the inhibition of cell proliferation by Bortezomib.Conclusion：Combination of 3-MA and Bortezomib increases human breast cancer cell death.

关 键 词：蛋白酶体抑制剂 自噬抑制剂 人乳腺癌细胞株 MCF-7 

分 类 号：R737.9[医药卫生—肿瘤]