磷脂酰肌醇蛋白聚糖-3在乳腺癌组织中的表达及其意义  

Expression of glypican-3 in breast cancer and its significance

作　　者：周栋[1] 徐亮[1] 李建国[1] 

机构地区：[1]江苏省常熟市中医院(新区医院)普通外科,江苏常熟215500

出　　处：《中国普通外科杂志》2011年第11期1216-1220,共5页China Journal of General Surgery

摘　　要：目的探讨磷脂酰肌醇蛋白聚糖-3(glypican-3)在乳腺癌组织中的表达及其与乳腺癌临床病理参数的关系。方法采用RT-PCR方法检测52例乳腺癌组织及其癌旁组织中glypican-3基因的表达,计算其阳性率;并用Western blot方法检测该基因在乳腺癌组织及其癌旁组织中的蛋白表达。结果 glypican-3 mRNA在乳腺癌组织中表达率明显低于癌旁组织(P<0.05);在有淋巴结转移者中的表达率明显低于无淋巴结转移者(P<0.05);癌组织分化程度越低,glypican-3 mRNA的表达率越低(P<0.05);但其与患者的年龄、肿瘤的大小无关(P>0.05)。glypican-3蛋白在乳腺癌组织中表达亦明显低于癌旁正常组织(P<0.05),在有淋巴结转移者中的表达量明显低于无淋巴结转移者(P<0.05),癌组织分化程度越低,glypican-3蛋白的表达量越低(P<0.05)。结论 glypican-3在乳腺癌组织中的低表达可能与乳腺癌的发生发展及侵袭转移有关。Objective To investigate glypican-3 expression in breast cancer tissue and its correlation with elinicopathological factors of breast cancer. Methods The glypiean-3 gene expressions in the breast cancer tissues and adjacent normal tissues from 52 patients we detected with RT-PCR method, and then their positive rates were determined. The glypican-3 protein expressions in the breast cancer tissues and adjacent normal tissues were also detected by Western blot technique. Results The expression rate of glypican-3 mRNA of the breast cancer tissue was significantly lower than that of the adjacent normal tissue （ P 〈 0.05 ） , and of the patients with lymph node metastasis was significantly lower than that of the patients without lymph node involvement （ P 〈 0.05 ）. Furthermore, the less differentiated the cancer, the lower the expression rate of glypican-3 mRNA （ P 〈 0.05 ） , but the g｜ypican-3 gene expression was unrelated to the age and tumor size of patients （ P 〉 0.05 ）. The expression level of glypican-3 protein of the breast cancer tissue was significantly lower than that of the adjacent normal tissue （ P 〈 0.05 ） , and of the patients with lymph node metastasis was significantly lower than that of the patients without lymph node involvement （P 〈0.05 ）. A poorer differentiation of the cancer tissue was correlated with lower expression level of glypican-3 protein （P 〈 0. 05 ）. Conclusions The low expression state of glypican-3 may probably be associated with the occurrence and development, and as well as invasion or metastasis of breast cancer.

关 键 词：乳腺肿瘤/病理学 磷脂酰肌醇蛋白聚糖-3 RT-PCR WESTERN BLOT 

分 类 号：R737.9[医药卫生—肿瘤]