纳米炭吸附紫杉醇对胃癌淋巴结内肿瘤细胞凋亡的影响  

Effect of paclitaxel-loaded carbon nanoparticles on apoptosis of tumor cells in lymph nodes of gastric cancer

作　　者：李伟[1] 徐亮[1] 姬俊成[1] 夏冬[1] 魏仁志[1] 

机构地区：[1]泸州医学院附属医院胃肠外科,四川泸州646000

出　　处：《中国普通外科杂志》2011年第11期1230-1234,共5页China Journal of General Surgery

基　　金：四川省科技厅基金资助项目(2006J13-164)

摘　　要：目的探讨纳米炭(CNP)吸附紫杉醇(PA)靶向化疗药物的作用机制及疗效。方法选取30例胃癌患者纳入研究,分为试验组和对照组,每组各15例。试验组术中先以纳米炭吸附紫杉醇行淋巴靶向化疗后再行手术,对照组直接手术。术毕即取胃癌周围淋巴结送病检,将病检证实有癌转移的淋巴结行TUNEL法检测细胞凋亡,用免疫组化法检测Bcl-2及p16的阳性表达。结果试验组淋巴细胞的凋亡指数[(28.6±5.2)]%明显高于对照组[(7.0±1.9)]%(P<0.05);试验组及对照组淋巴细胞第一站与第二站间Bcl-2和p16阳性表达均无统计学差异(均P>0.05);试验组淋巴细胞Bcl-2的阳性表达率(1 6.7%)明显低于对照组(5 6.7%)(P<0.0 5),而p1 6的阳性表达率(63.3%)明显高于对照组(23.3%)(P<0.05)。结论 PA-CNP混悬液能靶向作用于胃癌转移淋巴结,使Bcl-2表达下降及p1 6表达增加,从而促进细胞凋亡。Objective To investigate the efficacy of pacfitaxel-loaded carbon nanoparticles （PA-CNP） , the targeted chemotherapeutic drug, on apoptosis of tumor cells in lymph nodes of gastric cancer and its mechanism. Methods Thirty patients with gastric cancer were selected to participate in this study. The patients were assigned into observation group and control group with 15 cases in each group. The patients of the observation group were intraoperatively given lymphatic targeting chemotherapy of paclitaxel-loaded carbon before surgical resection, while the patients of the control group underwent surgery for gastric cancer directly. The regional lymph nodes of gastric cancer underwent pathological examination after surgery. The apoptosis in the metastatic nodes confirmed by pathology were detected with TUNEL assay, and the positive expression of Bcl-2 and p16 were determined by immunohistochemical staining. Results The apoptotic index of lymphocytes of the observation group [ （ 28.6± 5.2 ） % ] was significantly higher than that of the control group [ （7.0 ±1. 9 ） % （ P 〈 0. 05 ）. The positive expression rate of Bcl-2 and p16 of lymphocytes between the first and the second station lymph nodes had no difference in either the observation group or control group （ both P 〉 0. 05 ）. The positive expression rate of Bcl-2 of lymphocytes in the observationgroup （ 16.7% ） was significantly lower than that in the control group （ 56.7% ） （ P 〈 0.05 ） , while the positive expression rate of p l 6 of lymphocytes in the observation group （63. 3 % ） was significantly higher than that in the control group （ 23. 3% ） （ P 〈 0. 05 ）. Conclusions The paclitaxel-loaded carbon nanoparticles suspension can target the metastatic nodes of gastric cancer to decrease Bcl-2 and increase p16 expression, and thereby promote apoptosis.

关 键 词：胃肿瘤/药物疗法 纳米炭 紫杉醇 细胞凋亡 Bcl-2 P16 

分 类 号：R735.2[医药卫生—肿瘤]