肺癌组织中嗜酸细胞浸润及白细胞介素5表达的意义  

Eosinophilic infiltration of lung cancer tissue and its correlation with the expression of interleukin-5

作　　者：韩翔[1] 张培[2] 王娟[1] 贺蓓[1] 

机构地区：[1]北京大学第三医院呼吸科,100191 [2]北京大学第三医院中心实验室,100191

出　　处：《中华医学杂志》2011年第14期956-960,共5页National Medical Journal of China

摘　　要：目的 探讨肺癌组织中嗜酸细胞浸润与白细胞介素5（IL-5）表达的意义.方法 采用组织化学变色酸2R特殊染色法标记45例肺癌患者癌及癌旁组织中的嗜酸细胞,观察嗜酸细胞浸润情况;同时应用免疫组化的方法检测IL-5的表达;研究其与临床及病理分型的关系.结果 45例患者肺癌组织中17例（37.8%）存在嗜酸细胞的高浸润,在嗜酸细胞高浸润病例肺癌组织中的嗜酸细胞计数较癌旁组织明显增高（P＜0.05）,NSCLC较SCLC癌组织中嗜酸细胞高浸润的比例明显增高（P＜0.05）;45例肺癌组织中41例（91.1%）IL-5强阳性表达,4例（8.9%）阳性表达,且均为癌细胞胞质表达,在癌旁组织中呈阴性或低表达,肺癌组织与癌旁组织中的IL-5表达差异有统计学意义（P＜0.05）;肺癌组织中嗜酸细胞浸润的数量与癌细胞中IL-5的表达无明显相关性（r=0.026,P＞0.05）.结论 部分肺癌患者肿瘤组织内存在嗜酸细胞高浸润,在NSGLC更为多见.肺癌细胞内普遍存在IL-5高表达,肺癌细胞内IL-5高表达与肺癌组织中嗜酸细胞的浸润量无明显相关性.Objective To study the correlation of the eosinophilic infiltration with the expression of Interleukin-5 （IL-5） in tissue specimens from the patients with lung cancer. Methods Eosinophilic infiltration was detected by chromotrope-2R histochemical staining. And the level of IL-5 was detected by immunohistochemistry in 45 lung cancer tissues and 36 corresponding normal lung tissues. Results Highlevel tissue eosinophilic infiltration was found in 17 of 45 （37. 8% ） cases of lung cancer. In cases with high-level tissue eosinophilic infiltration, the count of eosinophil in tumor tissues was significantly higher than that in corresponding normal lung tissue （ P ＜0. 05 ）. In non-small cell lung cancer （NSCLC）, the rate of high-level tissue eosinophilic infiltration cases was significantly higher than that of small cell lung cancer （SCLC） （P ＜ 0. 05 ）. IL-5 was expressed at a high level in all 45 cases of lung cancer and at a low level in corresponding normal lung tissues. Eosinophilic infiltration was not correlated with the expression of IL-5 in lung cancer tissues （r = 0. 026, P ＞ 0. 05）. Conclusion High-level eosinophilic infiltration of tumor tissues is found in some cases of lung cancer. Compared with SCLC, the rate of high-level tissue eosinophilic infiltration cases of NSCLC is significantly elevated. There is no correlation between the eosinophilic infiltration and the expression of IL-5 in lung cancer tissues.

关 键 词：肺肿瘤 嗜酸细胞 白细胞介素5 

分 类 号：R734.2[医药卫生—肿瘤]