阿尔茨海默病患者的脑脊液tau蛋白及Aβ1-42水平  

Cerebrospinal fluid biomarkers for early diagnosis of Alzheimer disease

作　　者：单守勤[1] 赵权 张立营[1] 孙英姿[1] 

机构地区：[1]济南军区青岛第二疗养院,青岛266071 [2]济南军区青岛401医院检验科

出　　处：《中国神经精神疾病杂志》2011年第11期691-693,共3页Chinese Journal of Nervous and Mental Diseases

基　　金：全军保健专项课题项目(编号:10BJ215)

摘　　要：目的探讨脑脊液(cerebrospinal fluid,CSF)中tau蛋白及β-淀粉样蛋白1-42(Aβ1-42)对诊断阿尔茨海默病(Alzheimer’s disease,AD)的早期诊断价值,寻找AD理想的生物学标志。方法采用酶联免疫吸附法检测36例AD、24例血管性痴呆患者(vascular dementia,VD)和26名正常对照者(对照组)CSF中tau蛋白及Aβ1-42浓度的变化。结果 CSF中tau蛋白平均浓度:AD组(336±126)ng/L,VD组(183±96)ng/L,对照组(98±54)ng/L,AD组CSF中tau蛋白浓度明显高于对照组,差异有统计学意义(P<0.05);CSF中Aβ1-42平均浓度:AD组(341±113)ng/L,VD组(457±132)ng/L,对照组(502±167)ng/L,AD组CSF中Aβ1-42浓度明显低于对照组,差异有显著性意义(P<0.05)。结论脑脊液Aβ1-42、tau蛋白浓度的变化,不仅对诊断阿尔茨海默病具有较高的敏感性和特异性,而且亦可作为阿尔茨海默病与血管性痴呆鉴别诊断的生物学指标。Objective To investigate the cerebrospinal fluid （CSF） levels of tau protein and [3-amyloid peptide1-42（Aβ1-42） in the patients with Alzheimer disease（AD） and their diagnosetic value for AD. Methods The subjects were 36 patients with AD and 26 controls, includeing 24 patients with vascular dementia（VD） and 26 normal controls （NC）. The CSF levels of tau protein and Aβ1-42 were measured with ELISA. Results The mean CSF levels of tau protein were （336 ± 126） ng/L in AD patients, （183 ± 96） ng/L in VD, and （98 ± 54） ng/L in NC, respectively, and the difference between AD and NC was statistically significant（P 〈 0.05）. The mean CSF levels of Aβ1-42 were （341 ± 113）ng/L in AD, （457 ± 132）ng/L in VD, and （502 ± 167） ng/L in NC, and the differentce between AD and NC was statistically significant （P 〈 0.05）. Conclusions Cerebrospinal fluid Aβ1-42, tau protein and tau protein are useful biomarkers for the diagnosis of AD with high sensitivity and specificity, and can be used to differentiate AD and VD.

关 键 词：阿尔茨海默病tau蛋白 β-淀粉样蛋白1—42酶联免疫吸附实验 

分 类 号：R749.16[医药卫生—神经病学与精神病学]