SFRP4抑表达对HT-29细胞侵袭能力的影响  

The Effect of SFRP4 on Invasive Ability of HT-29

作　　者：李晓虹[1] 杨海燕[1] 蔡铭泉[1] 刘凯华[1] 许林[1] 

机构地区：[1]厦门大学附属第一医院肿瘤中心,福建厦门361003

出　　处：《现代生物医学进展》2011年第22期4215-4217,4208,共4页Progress in Modern Biomedicine

摘　　要：目的:观察SFRP4对HT29细胞侵袭转移能力的影响。方法:以HT29细胞为研究对象,SFRP4 siRNA通过脂质体转染HT29细胞,western blot检测HT29细胞中Wnt、细胞核β-Catenin蛋白的表达、elisa法检测细胞培养上清MMP-2、MMP-9的含量,Transwell小室观察成的转移侵袭能力。结果:干扰SFRP4后能显著降低HT29细胞中Wnt、细胞核中β-Catenin蛋白的表达、细胞培养上清MMP-2、MMP-9的含量,(P<0.01),降低HT29细胞的转移侵袭能力(P<0.01)。结论:抑制SFRP4表达能抑制HT29细胞的转移侵袭,其机制可能与抑制HT29细胞Wnt信号通路有关。Objective： To study the effect of SFRP4 on invasive ability of HT29. Methods： HT29 cells were divide into control, non sense, SFRP4 siRNA groups. The effects of SFRP4 siRNA on HT-29 cells invasive ability in vitro was measured by Boyden chamber invasive models. Contents of Wnt, β -Catenin, MMP-2, MMP-9 protein were detected by western blot and ELISA methods, respectively. Results： In SFRP4 siRNA group, contents of Wnt, β -Catenin,MMP-2 and MMP-9 protein were decreased markedly （P〈0.01）, the invasive ability was decreased markedly（P〈0.01）, too. Conclusion： SFRP4 could increase the invasive ability of HT-29 cell.

关 键 词：SFRP4 MMP-9 MMP-2 β—Catenin 

分 类 号：R730.231[医药卫生—肿瘤]