外源激素对不同后熟程度西洋参种子种胚成苗的影响  

Eeffects of Exogenous Hormones on American Ginseng Embryos with Different After-ripening into Seedlings

作　　者：秦公伟[1] 张力[1] 晋海军[1] 曹小勇[1] 胡选萍[1] 周娜 

机构地区：[1]陕西理工学院生物科学与工程学院,陕西省资源生物重点实验室,陕西汉中723001 [2]南郑县高台中学,陕西南郑723113

出　　处：《种子》2011年第11期41-44,共4页Seed

基　　金：陕西省教育厅重点实验室重点科研计划项目(编号:09JS047)

摘　　要：以西洋参种子为材料,研究了6-BA或NAA与GA3组合及培养基中赤霉素灭菌方式对不同后熟程度种胚无菌苗培养的影响,筛选无菌苗培养的最佳培养基。结果表明:(1)在培养基中,同一GA3浓度下,添加NAA比6-BA更能促进种胚萌发,且形成的无菌苗子叶不衰老;(2)在同一培养基中,后熟程度高的种胚比后熟程度低的种胚萌发率高,长势好;(3)赤霉素应采取过滤灭菌方式加入培养基;(3)无菌苗培养最佳培养基为MS+2 mg/L NAA+5 mg/L GA3。In this paper, American ginseng seeds were used to study the effects of GA3 combined with 6-BA or NAA and GA3 sterilization methods on the embryos with different after-ripening into seedlings, and the best medium for aseptic seedlings culture were selected. The results showed that ： （ 1 ） when NAA was added, there was higher germination rate on medium containing the same GA3 concentration, and the cotyledons of aseptic seedlings was not aging. （2） the embryos with higher degree of after-ripening had better germination and growth than the lower degree after-ripening embryos in the same medium. （3） the better medium for aseptic seedlings culture was MS ＋ 2 mg/L NAA ＋ 5 mg/L GA3.

关 键 词：西洋参 种胚 无菌苗 

分 类 号：S567.51[农业科学—中草药栽培]