CD_(14) mRNA的表达在急性脑出血肠-肝轴紊乱中的作用及星蒌承气汤的干预研究  

Expression of CD_(14) mRNA in Intestine-liver Axis Disorder during Acute Cerebral Hemorrhage and Effects of Xinglouchengqi Decoction

作　　者：赵海滨[1] 唐杰[1] 马立华[2] 王威[1] 王帅[1] 张秀静[1] 

机构地区：[1]北京中医药大学第三附属医院,北京市100029 [2]北京藏医院,北京市100029

出　　处：《中国康复理论与实践》2011年第11期1031-1034,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金(81072772)

摘　　要：目的研究白细胞分化抗原14(CD14)在急性脑出血应激性肝损害内毒素信号转导通路中的作用,以及星蒌承气汤的干预作用。方法采用大脑注射Ⅶ型胶原酶法建立SD大鼠急性脑出血模型,随机分为正常组、单纯脑出血组、脑出血痰热腑实证组和星蒌承气汤组,分别分为24 h、48 h、72 h 3个时相亚组,每个亚组8只。实时荧光定量聚合酶链反应(PCR)观察肝、肠系膜淋巴结组织CD14 mRNA表达,并观察肝HE染色结果。结果脑出血急性期肝、肠系膜淋巴结组织CD14 mRNA表达升高(P<0.05),星蒌承气汤能明显减轻肝组织病理损伤。结论 CD14在介导肠源性内毒素性肝损害中起重要作用,星蒌承气汤对脑出血急性期痰热腑实证肝脏具有一定的保护作用。Objective To investigate the influence of CD14 on lipopolysaccharide（LPS） signal transduction pathway during the hepatic stress injury in acute cerebral hemorrhage,and explore the protection mechanism of Xinglouchengqi decoction.Methods The model of acute cerebral hemorrhage was established with injection of collagenase Ⅶ into internal capsule of cerebrum.The rats were randomly divided into blank control group,cerebral hemorrhage group,syndrome of phledm-heat and fu-organ excess model group and Xinglouchengqi group.Each group was divided into 3 time sub-groups（24 h,48 h,72 h）.The expression of CD14 mRNA in liver tissue and mesenteric lymph nodes were observed by real-time quantitative polymerase chain reaction.Results The expression of CD14 mRNA was higher in model-group than blank control group（P〈0.05）.Xinglouchengqi decoction could reduce liver pathological injury significantly.Conclusion CD14 plays an important role in endotoxin-mediated liver injury.Xinglouchengqi decoction can protect the liver in cerebral hemorrhage.

关 键 词：白细胞分化抗原14 急性脑出血 应激性肝损害 痰热腑实证 

分 类 号：R743.34[医药卫生—神经病学与精神病学]