RP-HPLC测定香椿叶中芸香苷的含量  

RP-HPLC Determination of Rutin in the Leaf of Toona Sinensis

作　　者：夏国华[1] 童馨苇[1] 丁妍[2] 杨欢[1] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]解放军第三五九医院药械科,江苏镇江212001

出　　处：《南京中医药大学学报》2011年第6期587-588,共2页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家中医药管理局中药释药系统重点研究室开放课题(2011NDDCM01002);江苏大学科研立项资助项目(10A238);江苏大学高级专业人才科研启动基金(11JDG073)

摘　　要：目的建立1种测定香椿叶中芸香苷含量的反相高效液相色谱法,为其内在质量控制提供参考。方法采用Thermo ODS Hypersil column色谱柱(4.6mm×150mm,5μm),流动相为乙腈-0.1%甲酸水溶液(16∶84),流速为1.0mL/min,检测波长为254nm,柱温为25℃。结果在以上色谱条件下,供试品溶液中的芸香苷与干扰成分完全分离,其在0.0104 4～0.261 0mg/mL范围内线性关系良好(r=1),重复性实验RSD=1.77%(n=6),平均加样回收率为96.9%～98.7%(n=3),RSD为3.56%～4.42%;试验测得2个批次香椿叶中芸香苷的含量分别为(0.673±0.010)mg/g和(0.498±0.005)mg/g。结论该方法准确,快速,重复性好,可用于香椿叶中芸香苷的含量测定。OBJECTIVE To establish a reversed phase-high performance liquid chromatography（RP-HPLC） method for the quantization of rutin in Toona Sinensis Folium with the purpose of providing a reference for its quality assessment.METHODS Thermo ODS Hypersil column（4.6 mm×150 mm,5 μm） was employed for the analysis.Mobile phase was composed of acetonitrile and water containing 0.1% formic acid at the ratio of 16 to 84.Flow rate was set at 1.0 mL/min,wave length for detection was 254 nm and column temperature was 25 ℃.RESULTS Under the above chromatographic conditions,rutin has been separated from other interferences.A strong linear relationship between peak area and concentration of rutin was observed within the range of 0.010 44~0.261 0 mg/mL（r=1）.The repeatability was found to be 1.77%（n=6） and recovery of rutin was from 96.9% to 98.7%（n=3）.In addition,the method was also used for two batches of samples and the contents were（0.673±0.010） mg/g and（0.498±0.005） mg/g,respectively.CONCLUSION An efficient and reliable RP-HPLC-DAD method was established,which can be used for routine analysis of rutin in Toona Sinensis Folium and to assist in quality control of this herb.

关 键 词：香椿 香椿叶 芸香苷 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]