大型海藻DNA与RNA同步抽提技术比较  

Comparison of Methods for Simultaneous Extraction of DNA and RNA from Seaweeds

作　　者：易乐飞[1,2] 李信书[2] 阎斌伦[1] 

机构地区：[1]淮海工学院江苏省海洋生物技术重点实验室,江苏连云港222005 [2]淮海工学院海洋学院,江苏连云港222005

出　　处：《水产科学》2011年第11期693-697,共5页Fisheries Science

基　　金：江苏省海洋生物技术重点实验室开放课题(2008HS004);淮海工学院自然科学研究项目(2010150023)

摘　　要：海藻含有的多糖、多酚等物质容易与核酸(DNA与RNA)结合形成不溶于水的复合物,干扰了后续的酶反应,制约了海藻核酸抽提。但抽提高质量核酸却是海藻分子生物学研究的第一步,因此比较了2种同步抽提技术对大型海藻DNA与RNA同步抽提的效果。应用TRIZOL法和CTAB+LiCl法同步抽提了3种海藻的DNA与RNA,接着应用紫外吸收、电泳、PCR、RT-PCR和荧光定量RT-PCR等检测核酸质量。结果显示2种方法均能获得较高纯度的总RNA,总RNA质量完全适合后续的反转录等反应;但在DNA抽提方面CTAB+LiCl法能获得更高质量的DNA。CTAB+LiCl法是一项适用于大型海藻的DNA与RNA同步抽提技术。The primary step in algal molecular biology is extraction of nucleic acids（DNA and RNA） from seaweeds containing high levels of polyphenols and polysaccharides which often bind to and/or co-precipitate with nucleic acids and interfere with subsequent enzymatic manipulation of their nucleic acids.Therefore,the effects of two methods,TRIZOL method and CTAB＋LiCl method,on simultaneous extraction of DNA and RNA from three sea weed species were studied,and the quality of nucleic acids was assayed by UV absorption,electrophoresis,PCR,RT-PCR and fluorescence real-time PCR.The results showed that higher quality of DNA was extracted by CTAB＋LiCl method than by TRIZOL,though the high-quality of total RNA was extracted by both methods.So the CTAB＋LiCl method was an inexpensive method for simultaneous extraction of DNA and RNA from seaweeds,and of general applicability to most seaweed species.

关 键 词：CTAB TRIZOL RNA DNA 同步抽提 海藻 

分 类 号：S917[农业科学—水产科学]