Deferiprone对染铝小鼠肾脏ATP酶、黄嘌呤氧化酶的影响  

Effect of Deferiprone on Activities of Kidney ATPase and Xanthine Oxidase of Aluminuminduced Mice

作　　者：赵慧娟[1] 李宁[1] 刘萍[2] 

机构地区：[1]滨州医学院卫生管理学院,山东烟台264003 [2]山东大学公共卫生学院,山东济南250012

出　　处：《食品与药品》2011年第11期388-391,共4页Food and Drug

摘　　要：目的探讨1,2-二甲基-3-羟基-4-吡啶酮(DFP)对AlCl3染毒小鼠肾脏三磷酸腺苷酶(ATPase)、黄嘌呤氧化酶(xanthine oxidase,XO)的影响。方法 50只健康昆明种小鼠,按体重随机分为5组:阴性对照组、铝染毒组、DFP低、中、高剂量组。小鼠经三氯化铝水溶液[200 mg/(kg·d)]灌胃4周染毒后,DFP低、中、高剂量组分别灌胃DFP水溶液[17.58,35.15,70.30 mg/(kg·d)]1周。测定小鼠肾脏铝含量和ATP酶、黄嘌呤氧化酶活性。结果三氯化铝染毒后小鼠肾脏铝含量,铝染毒组,低、中、高剂量组均高于阴性对照组(P<0.01,P<0.05);低、中、高剂量组比铝染毒组有所降低(P<0.01)。肾脏黄嘌呤氧化酶活性比较,铝染毒组高于阴性对照组(P<0.01),DFP各组均低于铝染毒组(P<0.01)。比较各组小鼠肾脏Na+K+-ATP酶及Ca2+Mg2+-ATP酶活性,铝染毒组均低于阴性对照组(P<0.01);低、中、高剂量组均高于铝染毒组(P<0.01)。结论口服DFP 1周可以促进肾脏铝排出,并能恢复ATP酶活性,降低黄嘌呤氧化酶活性。Objective To investigate the effect of deferiprone on the activities of kidney ATPase and xanthine oxidase of aluminum-induced mice.Methods Fifty healthy Kunming mice were randomly divided into 5 groups by body weight： negative control group,aluminum-induced group,deferiprone low-dose group,deferiprone medium-dose group,deferiprone high-dose group.The mice were treated by lavage with AlCl3 solution（200mg/（kg·d） AlCl3）for 4 weeks.Then the low,medium and high-dose groups were intragastrically administered with deferiprone（17.58,35.15,70.30mg/（kg·d） deferiprone,respectively）for 1 week.The content of aluminum and activities of ATPase and xanthine oxidase in kidney tissues were determined.Results The aluminum contents of aluminum-induced group and deferiprone low,medium and high-dose groups in kidney tissues were higher than negative control group（P〈0.01,P〈0.05）,but the aluminum contents of three deferiprone-treated groups were lower than aluminum-induced group（P〈0.01）.The activity of kidney xanthine oxidase in aluminum-induced group was higher than negative control group（P〈0.01）,whereas the activities of kidney xanthine oxidase in three deferiprone-treated groups were lower than aluminum-induced group（P〈0.01）.The activities of kidney Na＋K＋-ATPase and Ca2＋Mg2＋-ATPase in aluminum-induced group were lower than negative control group（P〈0.01） and three deferiprone-treated groups（P〈0.01）.Conclusion Deferiprone taken orally for 1 week in aluminum-induced mice can promote the discharge of kidney Al,recover the activity of ATPase and reduce the activity of xanthine oxidase.

关 键 词：铝 1 2-二甲基-3-羟基-4-吡啶酮 肾脏 腺苷三磷酸酶 黄嘌呤氧化酶 小鼠 

分 类 号：R114[医药卫生—卫生毒理学]