2种色型黄粉虫酯酶同工酶的比较  

Comparison of esterase isozymes in two color varieties of Tenebrio molitor L.

作　　者：黄琼[1,2] 胡杰[3] 苟琳[4] 

机构地区：[1]四川农业大学林学院森林保护学省级重点实验室,四川雅安625014 [2]四川农业大学林学院长江上游林业生态工程四川省重点实验室,四川雅安625014 [3]四川农业大学经济管理学院,四川成都611130 [4]四川农业大学生命科学与理学院生物系,四川雅安625014

出　　处：《浙江大学学报（农业与生命科学版）》2011年第6期610-616,共7页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：四川省教育厅资助项目(10ZA05607ZZ022);国家级大学生创新性实验计划资助项目(101062624)

摘　　要：采用不连续聚丙烯酰胺凝胶垂直板电泳并结合分光光度法,对黄、黑2种色型黄粉虫(Tenebrio molitor L.)各发育期的酯酶同工酶酶谱及酶活性进行比较研究.结果表明:在2种色型黄粉虫的各发育期,共发现迁移率不同的酯酶同工酶酶带15条,这些酶带分布于5个不同的泳动区;黑色型的幼虫和成虫分别比黄色型的幼虫和成虫各多1条特征性酶带;同一色型黄粉虫各发育期酯酶同工酶的酶带数、酶活性强弱及迁移率也变化较大,卵期的酶带数最少(4条)、最弱,其中2条为卵期的特有性酶带.酶活性检测进一步表明:从卵至成虫期,黑色型黄粉虫的羧酸酯酶(CarE)、乙酰胆碱酯酶(AchE)和磷酸酯酶(ACP和AKP)活性均比黄色型黄粉虫高;同一色型黄粉虫各发育阶段的CarE、AchE及ACP和AKP活性也呈现明显的规律性变化,即:卵期<成虫期<蛹期<幼虫期.上述结果为黄粉虫的抗药性研究及品种选育积累了一定的基础数据.In order to provide some references for studying the genetic variation and selecting varieties of Tenebrio molitor,the esterase isozyme zymograms and activity at different developmental stages of the yellow-and black-color varieties of T.molitor were compared using vertical polyacrylamide gel electrophoresis and spectrophotometry.The results showed that 15 enzyme bands with different mobility were found at all,which were distributed in five zones.The larvae and adults of the black-color variety showed one more distinctive band compared with those of the yellow larvae and adults,respectively.Moreover,band numbers,migration rate of enzyme bands and strength of enzymatic activity at different developmental stages of both color varieties varied greatly.Compared with other developmental stage,band numbers of esterase isozyme were the least（only four bands） and enzymatic activity was the weakest at egg stage,and two of four bands were found only at this stage.The enzymatic activities of carboxylesterase（CarE）,acetylcholine esterase（AchE）,acid phosphatase（ACP） and alkaline phosphatase（AKP） in black-color variety were always higher than those in yellow-color variety at all stages from egg to adult.While in the same variety,The enzymatic activity of CarE,AchE,ACP and AKP varied in the order of egg stage adult stage pupal stage larval stage.The results above build up some underlying data for further studying the insecticide resistance and variety selection of the mealworm beetles.

关 键 词：黄粉虫 黄色型 黑色型 酯酶同工酶 酶谱 酶活性 

分 类 号：Q969.97[生物学—昆虫学] Q965