人纤维蛋白原α、β、γ基因的克隆和表达研究  

Study on the Cloning and Expression of Human Fibrinogen α,β,γ Gene

作　　者：黄星[1,2] 许健[3] 魏永伟[1] 章晓栋[1] 邓同乐[1] 孙静[1] 于涟[1] 

机构地区：[1]浙江大学动物预防兽医研究所,杭州310029 [2]广州倍绣生物技术有限公司,广州510000 [3]浙江中医药大学生命科学学院,杭州310053

出　　处：《科技通报》2011年第6期859-862,共4页Bulletin of Science and Technology

基　　金：国家自然基金(30800218);浙江省自然科学基金(Y2080889);浙江省教育厅重点项目(Z200908281)

摘　　要：纤维蛋白原(fibrinogen,Fg)是凝血因子I,在体内经凝血酶(thrombin)作用下转变为纤维蛋白,发挥其凝血和止血功能。本研究通过反转录的方法,从杭州人肝脏中成功克隆到人纤维蛋白原α、β、γ基因,并将其定向插入原核表达载体中,人纤维蛋白原α、β、γ基因经成功诱导获得表达,进一步纯化获得人纤维蛋白原α、β、γ蛋白。序列经比对,与Genbank上公布的序列同源性达97%～99%,这项研究为我国汉族人群纤维蛋白原基因核苷酸多态性和单倍型研究发挥一定作用,并且对纤维蛋白封闭剂的开发,提供了重要的产业化实验依据和技术基础。Fibrinogen is the blood coagulation factor I,with the catalysis of thrombin,it plays an important role in coagulation and hemostasis.Our rearch group successfully cloned fibrinogen α,β,γ gene by RT-PCR from human hepatic cells,then inseted it into prokaryotic expression vector,respectivelly.Expressed and purified of human fibrinogen α,β,γ protein are successful.The purified protein then was identified by SDS-PAGE and western blot.Our research result will promote the study of Chinese Han population fibrinogen gene polymorphism and haplotypes.The study provides an important experimental basis for the industrialization and technological base for the development of effective medical clinical trial of fibrin sealant.

关 键 词：纤维蛋白原 基因序列 纤维蛋白封闭剂 

分 类 号：Q786[生物学—分子生物学]