产气肠杆菌PSB28的解磷机理研究  

The Study of Phosphate Solubilization Mechanism of Enterobacter aerogenes PSB28

作　　者：伊鋆 高晓蓉[1] 安利佳[1] 

机构地区：[1]大连理工大学生命科学与技术学院基因工程实验室,辽宁大连116024

出　　处：《中国农学通报》2011年第27期245-249,共5页Chinese Agricultural Science Bulletin

基　　金：辽宁省博士启动基金项目"高效解磷真菌DUTX1解磷的分子生物学机制研究"(20091017)

摘　　要：解磷微生物在提高土壤肥力及促进作物对磷的吸收方面具有重要作用,明确其利用难溶性无机磷的本质在实际应用中具有重要的意义。分别在以Ca3(PO4)2和K2HPO4为唯一磷源的培养基中,检测了PSB28的解磷能力,并比较了生长动态和代谢产物的方面差异以分析其代谢难溶性无机磷的机理。结果表明,与含K2HPO4的培养基相比,在以Ca3(PO4)2为唯一磷源的培养基中菌体生长量较大但生长速率较慢,pH略高,呈逐渐降低趋势。HPLC测定结果发现,有机酸种类和含量变化明显,且酸性磷酸酶活性明显增强。因此菌株PSB28解磷的主要原因是有机酸的螯合作用,酸性磷酸酶起到间接作用。Phosphate-solubilizing bacteria（PSB） plays an important role in improving soil fertility and soil health by converting insoluble P forms to soluble forms accessible by plants,and it is necessary to know phosphate solubilization mechanism in order to apply.This study assessed the phosphate solubilizing ability of a high efficiency PSB strain,PSB28,and investigated the mechanism of phosphate solubilization by comparing growth conditions inoculated in medium Ca 3（PO 4） 2 as the sole phosphorus source with K 2 HPO 4 as the sole phosphorus source.The results showed that in the medium with Ca 3（PO 4） 2,logarithmic phase was longer,but the maximum concentration of biomass was larger.pH showed decreasing gradually,which was higher than that of K 2 HPO 4 as the sole phosphorus source.HPLC results showed both the sorts and the quantity of organic acids in Ca 3（PO 4） 2 medium were complicated than that of K 2 HPO 4 medium.The activity of acid phosphatase was a little higher.All the results suggested that organic acids excretion by PSB28 might be the main reason and acid phosphatase had an indirect effect on P solubilization.

关 键 词：解磷细菌 解磷能力 生长动态 有机酸 磷酸酶 

分 类 号：S144.9[农业科学—肥料学]