赫氏埃希菌抗体的体外特异性抑菌实验  

Studies on Specific Antimicrobial Effect in Vitro Caused by Antibody of E.hermannii

作　　者：陈煜[1] 高宏伟[2] 邓明俊[2] 吴振兴[2] 张彦明[3] 肖西志[2,3] 

机构地区：[1]福建农林大学生命科学学院,福州350002 [2]山东出入境检验检疫局,山东青岛266002 [3]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《中国农学通报》2011年第27期303-307,共5页Chinese Agricultural Science Bulletin

基　　金：中国检验检疫科学研究院基础研究发展项目"食品和饲料中三种细菌的易感性评价;分型技术及食品和饲料种源高通量鉴定技术"(2009JK009)

摘　　要：为研究赫氏埃希菌抗体在体外对赫氏埃希菌的抑制作用,制备了兔抗赫氏埃希菌多克隆抗体,并用ProteinG树脂抗体纯化柱进行纯化,纯化后的抗体与赫氏埃希菌混合,在不同的时间点对混合物进行扫描电镜分析和培养基生长分析。同时,用赫氏埃希菌伤口感染20只BALB/c小白鼠做赫氏埃希菌多克隆抗体的治疗效果分析。结果表明,赫氏埃希菌抗体在加入后的20min开始裂解细菌,并在80min后全部将赫氏埃希菌裂解。混合物的细菌生长分析表明没有活性的赫氏埃希菌存在。小鼠感染实验表明被赫氏埃希菌感染的小鼠在应用该抗体治疗后,恢复时间明显比未用抗体治疗的对照组快。因此,所制备的赫氏埃希菌多克隆抗体将能作为一种免疫治疗的方法应用于临床治疗。To study the specific antimicrobial effect in vitro caused by antibody of E.hemannii.The polyclonal rabbit antibody against E.hermannii was prepared and purified with Protein Gresin subsequently.Scanning electron microscope and nutrient agar media were used to evaluate the mixture of purified antibody and E.hermannii at different time points.Twenty BALB/c rats infected by E.hermannii through wound on their backs were used to evaluate the therapy effect of the prepared antibody of E.hermannii.The result showed that E.hermannii began to lyse at 20minutes and was disintegrated completely at 80minutes.In addition,there was no alive E.hermannii grew on nutrient agars where the mixture was plated.Infection assay against rats showed that the recovery time of infected rats which were treated by prepared antibody was shorter than those of without antibody introduced.Prepared polyclonal antibody against E.hermannii could be used as one of immune therapy methods for clinical cases caused by E.hermannii.

关 键 词：赫氏埃希菌 抗体的体外抑菌作用 免疫治疗 

分 类 号：R392.7[医药卫生—免疫学]