Wnt2与胃硬癌间质纤维化的关系及意义  

Role of Wnt2 in stromal fibrosis of scirrhous gastric cancer

作　　者：于艺[1] 胡祥[1] 高雪[2] 孙志刚[2] 

机构地区：[1]大连医科大学附属第一医院普外科,辽宁大连116011 [2]大连医科大学附属第一医院病理科,辽宁大连116011

出　　处：《外科理论与实践》2011年第3期256-259,共4页Journal of Surgery Concepts & Practice

摘　　要：目的:探讨胃硬癌中Wnt/β-catenin信号通路的活化及与间质纤维化的关系。方法:采用免疫组化染色SP方法,检测29例胃硬癌(胃硬癌组)、29例胃非硬癌(非硬癌组)及30例癌旁良性胃组织(对照组)中Wnt2及β-catenin的表达,应用苦味酸-天狼猩红染色方法检测Ⅰ型与Ⅲ型胶原纤维在以上三组中的表达。结果:Wnt2在胃硬癌和非硬癌组织中表达水平明显高于对照组(P<0.01)。胃硬癌组中β-catenin在细胞核中的表达明显高于非硬癌组及对照组,三组间均存在显著性差异(P<0.05)。Ⅰ型与Ⅲ型胶原的表达与β-catenin胞膜的表达呈负相关(P<0.01),与β-catenin胞质胞核的表达均呈正相关(P<0.01)。结论:Wnt/β-catenin信号通路的活化参与胃癌的发生发展,并可能参与调节胃硬癌间质纤维化的发生过程。Objective To investigate the expression of Wnt2 in different type of gastric carcinoma based on the cancer—stromal relationship and its effect on the stromal fibrosis of scirrhous gastric carcinoma.Methods Immunohistochemical method was used to detect the expression of Wnt2 and p-catenin in 29 cases of scirrhous gastric carcinoma（SC group）,29 cases of non-scirrhous gastric carcinoma（NSC group） and 30 adjacent non-tumour tissues（control group）.Sirius red staining was performed to assess the expression of typeⅠandⅢcollagen fibers in the above 3 groups. Results The levels of the expression of Wnt2,β-catenin,typeⅠandⅢcollagen were significantly higher in SC group and NSC group than those in control group（P0.01）.The expression of typeⅠandⅢcollagen was positively correlated with cytoplasma and nuclear expression ofβ-catenin but negatively correlated with cell membrane expression ofβ-catenin （P0.01）.Conclusions Wnt/β-catenin signaling pathway has a relationship with the pathogenesis of gastric cancer,and plays a role in the formation of scirrhous gastric carcinoma characterized by stromal fibrosis.

关 键 词：胃硬癌 WNT信号通路 间质纤维化 

分 类 号：R735.2[医药卫生—肿瘤]