肝细胞生长因子在胃癌病人血清中的表达及意义  

Serum hepatocyte growth factor in gastric cancer and its clinical significance

作　　者：凌跃新[1,2] 童赛雄[1,2] 锁涛[1,2] 陈竟文[1,2] 

机构地区：[1]复旦大学附属中山医院普通外科 [2]复旦大学普通外科研究所,上海200032

出　　处：《外科理论与实践》2011年第3期260-263,共4页Journal of Surgery Concepts & Practice

摘　　要：目的:肝细胞生长因子(hepatocyte growth factor,HGF)是一种多肽生长因子,其促进细胞增殖,诱导细胞迁移、侵袭及参与血管生成,在肿瘤发生发展过程中具有重要作用。本研究通过检测胃癌病人血清HGF水平,分析其与胃癌临床病理特征的关系,并与常用肿瘤标志物CA19-9、CEA进行比较,探讨胃癌病人血清中HGF的表达及其临床意义。方法:用酶联免疫吸附夹心法检测80例胃癌病人术前血清HGF、CA19-9、CEA水平,并观察60例病人根治术后血清HGF变化。结果:胃癌病人血清HGF水平(2.94±2.67)μg/L明显高于正常对照组(1.02±0.31)μg/L(P=0.002);分层分析发现,随着疾病的进展,血清HGF水平逐渐升高[Ⅰ期(2.19±2.12)μg/L,Ⅱ期(2.53±2.38)μg/L,Ⅲ期(3.92±3.45)μg/L,Ⅳ期(4.13±3.71)μg/L];Ⅲ期和Ⅳ期分别与Ⅰ期进行比较,均有显著差异(P<0.05);有淋巴结及肝脏转移者的血清HGF水平升高更为明显,P=0.016、0.009。根治术后血清HGF水平(2.17±1.36)μg/L比术前(2.73±1.65)μg/L明显降低(P<0.05)。另外,80例病人血清HGF、CA19-9和CEA的阳性率分别为80%、16.2%和5.0%,HGF阳性率明显较高(P<0.01)。结论:胃癌病人血清HGF水平明显高于正常对照组,且血清HGF的表达水平与胃癌淋巴结及肝脏转移呈正相关.血清HGF表达水平可作为反映胃癌生物学行为和判断预后的参考指标。Objective Hepatocyte growth factor（HGF） is a polypeptide growth factor,which can promote cell proliferation,and induce metastasis,invasiveness and angiogenesis.It plays an important role in tumor development.We measured serum concentrations of HGF in 80 patients with gastric cancer and compared them with various clinicopathological characteristics and conventional tumor markers,including carcinoembryonic antigen（CEA） and carbohydrate antigen 19-9（CA19-9）,for evaluating of the significance of serum HGF levels in gastric cancer patients. Methods Preoperative serum HGF levels were measured by an enzyme-linked immunosorbent assay（ELISA） system in 80 patients with gastric cancer and the levels of CEA and CA19-9 in the same serum sample were examined using the ELISA system.Furthermore,preoperative and postoperative serum concentrations of HGF in 60 patients with curative resection were measured.Results The mean value of serum HGF in 80 patients was significantly higher than that of healthy controls[（2.94±2.67）μg/L vs.（1.02±0.31）μg/L,P=0.002].An increased serum HGF level was associated with stage progression;serum HGF level in stageⅠpatients was significantly lower than those in stageⅢand stageⅣpatients [（2.19±2.12）μg/L,（3.92±3.45）μg/L and（4.13±3.71）μg/L,respectively],and statistically significant differences were found between the serum HGF level of stageⅠand those of stageⅢ（P0.05） and stageⅣpatients（P0.05）.Pathological factor analyses demonstrated that the patients with lymph node and liver metastasis presented significantly higher serum concentration of HGF（P=0.016 and P=0.009,respectively）.After operation,serum level of HGF in 60 patients with curative resection were lowered,a statistical difference was found between pre-and post-operative values（P0.05）.In comparison with conventional tumor markers for gastric cancer,serum HGF level was elevated in 64（80%） of 80 gastric cancer patients,and the serum CA19-9 and CEA levels were elevated in 1

关 键 词：胃癌 肝细胞生长因子 预后 

分 类 号：R735.2[医药卫生—肿瘤]