瘦素受体在乳腺癌中的表达及其与乳腺癌相关基因的相关性分析  

Expression of leptin receptor in breast cancer and its correlation with breast cancer related genes

作　　者：王丹[1] 陈宇鹏[1] 王珂 张艳 

机构地区：[1]北京大学基础医学院生物化学与分子生物学系 [2]人体解剖学与组织胚胎学系,北京100191

出　　处：《解剖学报》2011年第6期807-809,共3页Acta Anatomica Sinica

摘　　要：目的探讨瘦素受体(LEPR)在乳腺癌细胞系以及正常乳腺和乳腺癌标本中的表达情况,并分析LEPR的表达与雌激素受体(ER)、E-钙黏附分子(E-Cad)、c-Myc、细胞周期蛋白D1(cyclin D1)表达的相关性。方法采用RT-PCR和Western blotting方法检测不同的乳腺癌细胞系中LEPR的表达情况。提取45例乳腺癌以及15例正常乳腺组织标本的RNA(其中5例为配对的乳腺标本),在5例配对的乳腺癌及其正常对照中用RT-PCR的方法检测了LEPR的表达,并同时在以上所有的乳腺癌以及正常乳腺组织标本中通过实时定量RT-PCR检测LEPR、ER、E-Cad,c-Myc和cyclin D1的表达情况。利用SPSS 10.0统计学软件,分析LEPR与ER、E-Cad、c-Myc、cyclin D1表达的相关性。结果在7种乳腺癌细胞系中均检测到了LEPR的RNA和蛋白。通过配对的乳腺癌标本,发现LEPR在乳腺癌标本中的表达高于远端正常组织。通过统计学分析发现,在正常乳腺和乳腺癌组织中LEPR的表达与ER表达呈正相关,在乳腺癌组织中与E-Cad、c-Myc有统计学相关性,与cyclin D1无统计学相关性。结论 LEPR在乳腺癌细胞系中表达增高,且LEPR的表达在乳腺癌中与一些乳腺癌发生发展密切相关的基因的表达存在相关性。Objective To study the expression levels of leptin receptor（LEPR） in breast cancer cell lines, normal breast samples and malignant breast cancer samples, and to analyze the expression correlation between LEPR and estrogen receptor（ER）, E-cadherin （E-Cad）, c-Myc and cyclin D1 in order to provide mechanism clues for obesiety associated breast cancer. Methods Using RT-PCR and Western blotting, we examined the expression of LEPR in different breast cancer cell lines. The expression level of LEPR was analyzed by RT-PCR in 5 paired breast samples. In the meanwhile, The expression of LEPR, ER, E-Cad, c-Myc and cyclin D1 was analyzed using realtime PCR in 45 breast cancer samples and 15 normal breast samples. The correlation was analyzed between the expression of LEPR and the expression of ER, E- Cad, c-Myc and cyclin D1 using SPSS statistic software. Results LEPR mRNA and protein were expressed in all breast cancer cell lines tested in this study. The expression of LEPR in the breast cancer samples was higher than in the paired adjacent normal breast tissues. With the statistic analysis, the expression of LEPR was correlated with expression of ER in both normal and malignant breast samples. LEPR expression was also close correlated with E-Cad and c-Myc expression in the breast cancer samples, but not with cyclin D1 expression. Conclusion LEPR expresses in breast cancer cell lines. Compared to the normal breast tissues, the expression of LEPR in breast cancers is enhanced. Moreover, LEPR expression is correlated with some breast cancer related genes.

关 键 词：LEPTIN受体 乳腺癌 反转录-聚合酶链式反应 免疫印迹法 人 

分 类 号：R737.9[医药卫生—肿瘤]