糖尿病对大鼠股骨头生长板分化和成骨的影响  

Effect of diabetes on the differentiation and osteogenesis mineralization of femoral head growth plate in rats

作　　者：陈海英[1] 陈小萍[1] 郑建盛[1] 陈少强[2] 刘文革[3] 唐军民[4] 

机构地区：[1]福建莆田学院医学院基础医学部,福建莆田351100 [2]福建医科大学人体解剖学与组织胚胎学系,福州350003 [3]福建医科大学附属协和医院骨科,福州350003 [4]北京大学医学部人体解剖学与组织学胚胎学系,北京100191

出　　处：《解剖学报》2011年第6期825-831,共7页Acta Anatomica Sinica

基　　金：福建教育厅科研基金资助项目(JB08222)

摘　　要：目的探讨糖尿病对长骨发育的影响机制。方法建立速发型链脲佐菌素(STZ)糖尿病大鼠模型,分为糖尿病5周组(DM1)、10周组(DM2)及15周组(DM3),每组10只,另设正常5周组(CON1)、10周组(CON2)及15周组(CON3),每组10只,共60只。对各组大鼠股骨头生长板,光镜下作组织病理学分析及组织计量学测定;利用Van Gieson胶原纤维染色法观察胶原纤维沉积变化;墨汁灌注行边缘微血管密度测定;观察生长板血管内皮生长因子(VEGF)mRNA原位杂交表达强度并分析;采用RT-PCR技术,对蛋白聚糖的表达进行了观察。结果糖尿病15周组生长板厚度、生长板细胞柱密度均明显小于对照组(P<0.01);生长板钙化区胶原纤维明显增多;VEGFmRNA表达均高于正常组(P<0.01);边缘微血管密度增大,但渗透性大。15周蛋白聚糖的表达水平显著高于对照组。结论糖尿病大鼠股骨头生长板出现早闭。VEGF促进成骨矿化,糖尿病股骨头生长板边缘微血管密度的变化直接影响生长板软骨细胞的增殖分化与成骨矿化。Objective To study the effect of diabetes on development of long bones. Methods The diabetic model（STZ） of the rat was established. Rats were randomly divided into 3 normal groups：5-week（ CON1 ）, 10-week （CON2） and 15-week（CON3） and 3 diabetic groups-5-week（DM1）, 10-week（DM2） and 15-week（DM3）,10 rats each group. The change of collagen fiber depositing was observed by Van Gieson staining method. The microvaseular density was measured by the ink infusion method. The techniques of in situ hybridization and RT-PCR were used in this study in order to analyze the intensity of vascular endothelial growth factor （VEGF） mRNA expression and content of deeorin, respectively. Results The thickness and cellular density of the cell pillar in the growth plate were obviously lower in the 15-week diabetic group than those in the control group （ P 〈 0.05 ）. The content of collagen in the calcified zone of the growth plate was higher than that in the control group （P 〈 0. 05 ） , and the content of decorin decreased in the 15-week diabetic group（ P 〈 0. 01 ） , the intensity of VEGFmRNA expression increased （P 〈 0.01 ） , the mierovascular density increased. Conclusion The cellular proliferation, differentiation and its osteogenesis mineralization in the growth plate of the femoral head in the 15- week diabetic rats are developing during the course of the illness.

关 键 词：糖尿病 生长板 胶原纤维 蛋白聚糖 血管内皮生长因子 墨汁灌注 原位杂交 大鼠 

分 类 号：R587.2[医药卫生—内分泌]