稳定表达人Siat7e基因的MDCK细胞系的建立和全悬浮细胞的驯化  

Generation of a MDCK Cell Line to Suspension Culture by Stably Experessing Human Siat7eGene

作　　者：张妍[1] 张文俊[1] 李群辉[1] 刘晓文[1] 刘文博[1] 刘秀梵[1] 

机构地区：[1]扬州大学兽医学院农业部畜禽传染病学重点开放实验室,扬州225009

出　　处：《中国生物工程杂志》2011年第11期75-80,共6页China Biotechnology

基　　金：现代农业产业技术体系建设专项资金(nycytx-41-G07);国家"973"计划(2011CB505003))资助项目

摘　　要：目的:现有的禽流感疫苗生产的方法已经不能适应工业化大生产的需求,有必要开发全悬浮培养的细胞系来满足大生产的需求。方法:我们通过转染稳定表达Siat7e基因的真核表达载体对MDCK细胞进行改造,并经过后期的驯化,筛选适应于全悬浮培养的MDCK细胞。结果:成功筛选到能稳定表达Siat7e基因并能适应全悬浮生长的细胞系。结论:该细胞系具有潜在的应用价值,为MDCK细胞的培养以及工业化大生产疫苗提供参考。Object：Current influenza vaccine manufacturing have been unable to meet the demand of Industrialized production.Method： Anchorage-dependent MDCK cells were converted to anchorage-independent cells,capable of growing in suspension as a result of transfection with the human Siat7e gene（ST6GalNac V）.Result：Successfully conversioned a MDCK cell line stably experessing human Siat7e and they were capable of growing in suspension cultures.Conclusion：These results are critical for establishing large-scale suspension culture of MDCK cells as subsequent well as large-scale vaccine production.

关 键 词：MDCK Siat7e 全悬浮 疫苗生产 

分 类 号：Q786[生物学—分子生物学]