不同fimA型牙龈卟啉单胞菌降解上皮细胞间连接蛋白能力的初步研究  

Proteolysis of cellular adherence junction components of epithelial cells by Porphyromonas gingivalis with distinct fimA genotypes

作　　者：苗棣[1] 高雳[1] 赵蕾[2] 邓辉[1] 王韵[1] 郭淑娟[1] 吴亚菲[2] 

机构地区：[1]四川大学华西口腔医学院口腔疾病研究国家重点实验室,成都610041 [2]四川大学华西口腔医学院牙周科,成都610041

出　　处：《实用口腔医学杂志》2011年第6期769-772,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金资助项目(编号:30471890);高等学校博士学科点专项科研基金(编号:20070610066)

摘　　要：目的:研究不同fimA型牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)破坏上皮细胞间连接能力,探讨fimA基因型与致病力差异之间的关系。方法:应用不同fimA型P.g菌株ATCC 33277(Ⅰ型),WCSP115(Ⅱ型),WCSP1.5(Ⅲ型),W83(Ⅳ型),3501(Ⅴ型),4702(Ib型)分别与口腔上皮细胞系KB细胞在标准条件下共同孵育,于孵育后0.5、1和3 h,收集细胞蛋白,应用Westen-blot检测N-钙粘素(N-cadherin)和β-连环素(β-Catenin)的动态表达。结果:与对照组比较,各fimA型P.g对KB细胞N-cadherin和β-Catenin蛋白的表达均有明显的降解作用;其中ⅡfimA型组N-cadherin和β-Catenin蛋白的表达量低于其它fimA型组。结论:P.g可破坏上皮细胞间连接成分,破坏上皮细胞的完整性,而Ⅱ型fimA型P.g水解上皮细胞间粘合连接蛋白的能力强于其它fimA型。提示fimA基因型的不同可能是P.g的致病力差异的基因基础。Objective： To study the effects of Porphyromonas gingivalis（P.g） stains with different fimA genotypes on the proteolysis of cellular adherence junction components of epithelial KB cells.Methods： KB cells were challenged by P.g stains for different time periods and cell extracts were collected.Proteolysis of cellular adherence junction components,N-cadherin and β-Catenin were assayed by western-blots.Results： Degradation of N-cadherin and β-Catenin was detected after incubation of KB cells with different fimA genotypes of P.g,while the stain with type Ⅱ fimA genotype showed the most potent effect.Conclusion： P.g may impair the integrity of epithelium.P.g with type Ⅱ genotype has the most efficient ability to degrade the adherence junction components of epithelial cells.

关 键 词：牙龈卟啉单胞菌 fimA分型 KB细胞 钙粘素 连环素 

分 类 号：R781.4[医药卫生—口腔医学]