RasTG基因与胰腺癌的相关性研究  

Study on Relationship Between RasTG and Pancreatic Carcinoma

作　　者：龚军[1] 向光明[2] 郑振江[2] 谭春露[2] 刘续宝[2] 陈雁[3] 

机构地区：[1]四川省医学科学院四川省人民医院肝胆胰外科,四川成都610072 [2]四川大学华西医院肝胆胰外科,四川成都610041 [3]中国科学院上海生命科学研究院营养科学研究所,上海200031

出　　处：《中国普外基础与临床杂志》2011年第11期1169-1174,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：四川省科技厅应用基础研究基金资助项目(项目编号:03JY029-081-1)~~

摘　　要：目的体外观察高尔基嵌合Ras基因(RasTG基因)在胰腺癌组织中的表达情况和对人胰腺癌细胞株PANC-1的生长、增殖及细胞成瘤能力的影响,并探讨其作用机理。方法以人胰腺癌细胞株PANC-1为研究对象,构建含RasTG基因的慢病毒转染人胰腺癌细胞株PANC-1,利用RNA干扰技术干扰细胞内RasTG基因的表达,观察干扰后PANC-1细胞的增殖、转化和成瘤情况,并通过组织芯片技术检测胰腺导管癌及其癌旁组织中RasTG蛋白的表达情况。结果 ①RasTG无十分特异的组织表达谱,在脑、肝脏及肾上腺中表达相对较高;②RasTG在胰腺导管癌组织中的表达明显高于癌旁组织(P<0.05);③干扰RasTG基因后的人胰腺癌细胞株PANC-1的生长、增殖和转化能力均下降;④干扰RasTG基因后的人胰腺癌细胞株PANC-1的成瘤能力明显减弱,转染组的成瘤体积明显小于未转染组(P<0.05)。结论 RasTG基因在动物体内广泛分布;RasTG在胰腺癌组织中的表达比在癌旁组织中高;干扰RasTG基因的表达后对人胰腺癌细胞株PANC-1的增殖、转化和成瘤能力均有抑制作用,RasTG基因是正调节因子。Objective To study the expressions of Ras trapping to Golg i（RasTG） genes in pancreatic carcinoma tissues and to observe the growth,proliferation and the impact of tumors formation of human pancreatic cancer cells（PANC-1）,and to explore its mechanism.Methods Made PANC-1 as a target to research,transfected RasTG genes into PANC-1,used RNAi technology and observed the growth,proliferation and the impact of tumors formation of the cells.Meantime,contrasted the RasTG expressions between pancreatic ductal cancer and adjacent tissue by tissue microarray technology.Results ①The express ion of RasTG gen e in tissues was not very differential,which was higher in the brain,liver,an d adrenal gland.②The expression of RasTG protein in pancreatic ductal carcino ma was significantly higher than that in adjacent tissues（P0.05）.③After Ra sTG RNAi in PANC-1 cells,the ability of growth and proliferation were decreased. ④The ability of tumors formation in PANC-1 cells after RNAi was decreased,c a rcinoma＇s volume of transfected group was significantly smaller than that in the non-t r ansfected group（P0.05）.Conclusions RasTG gene is widely distributed in ani mals.RasTG protein in pancreatic carcinoma tissues is higher than that in adjacent tissues.The ability of proliferation,transformation and tumors formation in PA NC-1 cells after RNAi of RasTG gene are restrained,RasTG gene is a positive r egulatory factor.

关 键 词：RAS蛋白 RasTG基因 胰腺癌 

分 类 号：R735.9[医药卫生—肿瘤] R730.231[医药卫生—临床医学]