早期肝癌术后患者调节性T细胞数量及功能变化  

Quantity and Function of CD4^+CD25^+FOXP3^+ Regulatory T Cell Decreased after Operation in Hepatocarcinoma Patients

作　　者：王学范[1] 姜晓峰[1] 付庆才[2] 林琳[1] 魏云涛[2] 郭大伟[1] 梁健[1] 

机构地区：[1]中国医科大学附属第四医院普外科,辽宁沈阳110032 [2]辽宁省肿瘤医院肿瘤外科,辽宁沈阳110042

出　　处：《中国普外基础与临床杂志》2011年第11期1180-1183,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：辽宁省教育厅高等学校科研项目计划基金(项目编号:2008824;2009A729)~~

摘　　要：目的通过分析早期肝癌患者肝癌切除手术前、后外周血中CD4+CD25+FOXP3+调节性T细胞(Treg)数量及功能的变化,从免疫抑制角度探讨手术对肝癌患者免疫功能的影响。方法采集肝癌患者(病例组,15例)手术治疗前、后及正常人群(对照组,5例)外周血,提取淋巴细胞,行细胞外(CD4、CD25)及细胞内(FOXP3)染色,应用流式细胞仪分析Treg的数量及功能。结果 病例组CD4+CD25+T细胞和CD25+FOXP3+T细胞在术前外周血中所占比例均明显高于正常对照组〔(12.43±2.57)%比(5.56±1.02)%,(5.14±1.4)%比(2.18±0.83)%,P<0.05〕,病例组以上两种细胞在术后1周﹝(10.56±2.13)%,(4.28±1.08)%﹞较术前均有所下降,但差异无统计学意义(P>0.05),而术后2周〔(7.30±0.89)%,(3.43±0.83)%〕较术前下降明显(P<0.05)。病例组CD8+T细胞和CD4+CD25-T细胞在术前外周血中所占比例明显低于正常对照组〔(23.42±1.80)%比(29.22±2.26)%,(36.14±1.12)%比(43.69±2.78)%,P<0.05〕,病例组以上两种细胞在术后2周〔(27.15±1.71)%,(40.30±2.00)%〕较术前均明显升高(P<0.05)。对Treg与AFP进行相关性分析发现二者具有低度相关关系(r=0.48,P<0.05)。结论肝癌切除术能够在一定程度上改善肝癌患者的免疫功能,且Treg在肝癌的诊治及预后的判断中,可能具有一定辅助意义。Objective Through the analysis of quantitative and functiona l changes in peripheral blood CD4＋ CD25＋FOXP3＋ regulatory T cells（Treg） of e arly HCC patients before and after operation,to discuss the operation effect on the immune function from the aspect of immune suppression.Methods Extracted t he lymphocytes of peripheral blood in HCC patients before and after operation（ca se group,n=15） and normal people（control group,n=5 cases）,and an alyze the number and function of Treg by flow cytometer after extracellular（CD4,CD2 5） and intracellular（FOXP3） staining.Results CD4＋CD25＋ T cells and CD25＋FOXP3＋ T cells in preoperative peripheral blood in case group were signi ficantl y higher than those in control group（12.43±2.57）% vs.（5.56±1.02）%,（5.14±1.4）% vs.（2.18±0.83）%,P0.05）.These two cells decreased at 1 week after operation.（（10.56±2.13）%,（4.28±1.08）%）,but there was not statistically significant（P0.05）,they decreased significantly at 2 weeks after operation（（7.30±0.89）%,（3.43±0.83）%,P0.05）.CD8＋ T cells and CD4＋CD25-T cells in preoperative peripher al blood in case group were significan t ly lower than those in control group（（23.42±1.80）% vs.（29.22±2.26）%,（36.14±1.12）% vs.（43.69± 2.78）%,P0.05）,These two cells decreased significa ntly at 2 weeks after operation（（27.15±1.71）%,（40.30± 2.00）%）.The analysis on the Treg and AFP correlation found that they have low correlation（r=0.48,P0.05）.Conclusions The hepatectomy can improve the immune response o f HCC patient.Treg may have a certain auxiliary significance in the diagnosis, treatment and prognosis of patients with hepatocellular carcinoma.

关 键 词：肝癌 调节性T细胞 免疫调节 叉头状转录因子P3 

分 类 号：R735.7[医药卫生—肿瘤]