非霍奇金淋巴瘤血清蛋白质质谱分析  

Analysis of Serum Pyoteome Profiles of Non-Hodgkin's Lymphoma

作　　者：张志[1] 白寒松[1] 丁银环[2] 程小柯[1] 周靖泳[1] 韩丽英[1] 

机构地区：[1]泸州医学院附属医院血液科,四川泸州646000 [2]泸州医学院附属医院检验科,四川泸州646000

出　　处：《中国临床医学》2011年第5期711-713,共3页Chinese Journal of Clinical Medicine

摘　　要：目的:应用表面增强激光解析电离飞行时间质谱技术检测血清蛋白指纹图,通过差异蛋白组学筛选非霍奇金淋巴瘤(non-hodgkin’s lymphoma,NHL)特有的蛋白标记物,找出最佳的标志蛋白组合模式建立非霍奇金淋巴瘤诊断模型。方法:用Ciphergen公司生产的PBSⅡ/C型蛋白质指纹图谱仪和Au-Chpi金芯片采集50例NHL患者(NHL组)、50例淋巴结炎患者(淋巴结炎组)、50例健康志愿者(对照组)血清蛋白质指纹图谱,采用Biomaker Wizard3.1版分析软件筛选差异蛋白,建立NHL诊断模型。结果:NHL患者、淋巴结炎患者、健康志愿者血清蛋白质指纹图谱之间有4个标志蛋白在NHL患者血清中高表达,3个标志蛋白在NHL血清中低表达。分析系统筛选出质荷比(M/Z)4966、9278、6633、4222、9216、9237和7714标志蛋白建立NHL诊断模型,该模型对NHL的诊断灵敏度和特异性分别为92%和98%。结论:NHL与淋巴结炎组、对照组比较血清蛋白质表达具有显著差异,筛选其中差异蛋白质可用于建立NHL预测模型。Objective：To study the serum biomarkers of non-hodgkin＇s lymphoma（NHL）by surface enhanced laser desorption/ ionization time- of- flight mass spectrometry （SELDI- TOF MS）and establish NHL diagnostic model. Methods： The study included 50 patients with NHL （NHL group）. 50 normal controls （control group） and 50 poradenitis （poradenitis group） patientsForserum protein signature analysis, the SELDI- TOF-MS analysis was performed to screen the serum protein markersand the Au-Chpi golden chips were used for analysisA diagnostic model was developed with Biomaker pattern software3.1. Results： The data analysis revealed 150 differential expression peaks. Seven specific protein peaks with M/Z at4966, 9278, 6633, 4222, 9216, 9237 and 7714,were chosen to develop a NHL diagnostic model. When the model was blindly tested with the test set, it yielded a sensitivity of 92%,a specificityof 98%. Conclusions： Serum protein expression has obvious difference in NHL group, normal group and poradenitis group. Screening of differentially expressed biomarkers establish predictive models for the laboratory diaenosis of NHL.

关 键 词：非霍奇金淋巴瘤 表面增强激光解析电离飞行时间质谱 蛋白组学 

分 类 号：R733[医药卫生—肿瘤]