散发性胆囊癌组织hMLH1基因启动子甲基化检测及其临床意义  

Analysis and clinical significance of hMLH1 promoter methylation status and protein expression in patients with sporadic gallbladder carcinoma

作　　者：张国强 王晔恺 周吉航 卢海英[2] 

机构地区：[1]舟山医院,浙江舟山316004 [2]浙江海洋学院医学院,浙江舟山310031

出　　处：《中国卫生检验杂志》2011年第11期2668-2670,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:探讨散发性胆囊癌组织中错配修复基因hMLH1启动子甲基化状态和蛋白表达检测的临床意义。方法:收集我院胆囊癌患者47例,采用甲基化PCR和Envision免疫组化法检测其胆囊癌组织中hMLH1启动子甲基化状态和蛋白表达。结果:hMLH1启动子甲基化阳性率在肝脏浸润组间、淋巴结转移组间、Nevin分期之间差异均具有显著统计学意义(P<0.01或P<0.05)。hMLH1蛋白表达在hMLH1基因启动子组间、肝脏浸润组间、淋巴结转移组间、Nevin分期之间差异均具有显著统计学意义(P<0.01或P<0.05)。结论:散发性胆囊癌中启动子甲基化是hMLH1基因失活的原因之一,hMLH1的启动子甲基化和蛋白检测对评估胆囊癌浸润和转移具有重要意义。Objective：This study is to determimate the promoter methylation status and protein expressions of hMLH1 in patients with sporadic gallbladder carcinoma to identify the role in pathogenesis,development and classification of sporadic gallbladder carcinoma.Methods： 47 patients with sporadic gallbladder carcinoma were selected and the promoter methylation status and protein expressions of hMLH1 in carcinoma tissue were detected by MS-PCR and immunohistochemical techniques respectively.Results： The hMLH1 promoter methylation rate among liver invasion groups,lymph node metastasis groups,Nevin stages were significantly different（P0.01 or P0.05）.The hMLH1 protein expression among hMLH1 promoter methylation groups,liver invasion groups,lymph node metastasis groups,Nevin stages were significantly different（P0.01 or P0.05）.Conclusion： Promoter methylation in sporadic gallbladder carcinoma was one reason of the hMLH1 gene inactivation.The promoter methylation and protein detection of hMLH1 was of great value to evaluate the infiltration and metastasis of gallbladder carcinoma.

关 键 词：胆囊癌 HMLH1基因 HMSH2基因 免疫组化 

分 类 号：R735.8[医药卫生—肿瘤]