副溶血弧菌和霍乱弧菌合检增菌液pH值和盐度的研究  

Research on pH value and salinity of multipathogen enrichment broth for simultaneous growth of V.parahaemolyticus and V.cholera

作　　者：沈飚 胡兴娟 汪云泉 张文斌 

机构地区：[1]舟山出入境检验检疫局水产品实验室,浙江舟山316000 [2]纽迪希亚制药(无锡)有限公司产品开发部,江苏无锡214111

出　　处：《中国卫生检验杂志》2011年第11期2682-2684,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省重点国际合作研究项目(2009C14025)

摘　　要：目的:研究共同增菌液pH值和盐度对副溶血弧菌和霍乱弧菌生长的影响,找出副溶血弧菌和霍乱弧菌共增菌液的最佳pH值和盐度。方法:利用副溶血性弧菌、霍乱弧菌以及杂菌对共同增菌液的pH值和盐度进行实验,通过弧菌显色平板计数验证增菌效果,从而确定两种弧菌的共同增菌液pH和盐度。结果:副溶血性弧菌和霍乱弧菌合检增菌液的最佳条件为pH8.4,盐度2.1%。结论:增菌液pH值和盐度的改变对副溶血性弧菌和霍乱弧菌生长具有一定影响。Objective：To study the effect of different pH values and salinities of multipathogen enrichment broth on the growth of V.parahaemolyticus and V.cholera,and to find out the best pH value and salinity.Methods： V.parahaemolyticus,V.cholera and other bacteria were applied to determine the pH value and salinity,and the enrichment effect was evaluated by Vibrio chromogenic medium.Results： The enrichment broth with pH 8.4 and salinity 2.1% are the most suitable condition to V.parahaemolyticus and V.cholera.Conclusion： pH value and salinity of enrichment broth can influence the growth of V.parahaemolyticus and V.cholera.

关 键 词：副溶血性弧菌 霍乱弧菌 共增菌液 PH值 盐度 

分 类 号：R446.5[医药卫生—诊断学]