离子注入水稻细胞学效应研究  

Study on Cytological Effects of Rice Implanted with Ion Beam

作　　者：代西梅[1] 张佳佳[1] 杨旭[1] 黄群策[1] 

机构地区：[1]郑州大学河南省离子束生物工程重点实验室,河南郑州450052

出　　处：《郑州大学学报（理学版）》2011年第4期96-99,共4页Journal of Zhengzhou University:Natural Science Edition

基　　金：河南省自然科学基金资助项目;编号2008A208019;农业部农业公益性行业科研专项经费资助项目;编号200803034

摘　　要：以水稻品种紫粳二倍体和四倍体为实验材料,以低能氮离子束为诱变源,研究了离子束诱变对水稻根尖细胞染色体的影响.结果发现,低能离子束注入引起了水稻根尖分生区细胞有丝分裂异常,出现许多染色体畸变类型,包括后期染色体桥、落后染色体、染色体断片、中期染色体单价体等.染色体畸变率并不是随着剂量的增加呈现直线上升的趋势,而是有一定的摆动性,但总体趋势是注入剂量越大,畸变率越高.由此可见,离子注入引起水稻染色体异常是其发生遗传性变异的细胞学基础.The seeds of diploid and tetraploid rice were implanted by nitrogen ion beam.The cytological effects of the root tip cells were studied.The results showed that ion beam implantation could inhibit the survival rate and lots of types of chromosome aberration were observed in the mitosis of root tip cells of M0 generation,such as chromosome bridge,chromosome fragment,univalent and polyvalent.Chromosome aberration rate was not proportional to N＋ ion treatment dosages.But as a whole,the more of the dose,the higher of the chromosome aberration rate.The results proved that ion beam could cause abnormity of chromosomes,which might be the cytological reason that leading to the heritable mutations.

关 键 词：水稻 离子束注入 有丝分裂 诱变 染色体 

分 类 号：S121[农业科学—农业基础科学]