二十二碳六烯酸降低β淀粉样蛋白25-35致大鼠皮质神经元损伤  

Ducosahexaenoic acid reduces the cerebral cortical neurons injury induced by amyloid beta protein 25-35 in rats

作　　者：刘丽莉[1] 王永[1] 胡为民[1] 李世芳[1] 张记君[1] 

机构地区：[1]山西医科大学第二医院神经内科,山西太原030001

出　　处：《基础医学与临床》2011年第12期1346-1350,共5页Basic and Clinical Medicine

基　　金：山西省回国留学人员基金(2004-7-35)

摘　　要：目的观察二十二碳六烯酸(DHA)对β淀粉样蛋白25-35(Aβ25-35)致原代培养大鼠皮质神经元损伤的保护作用。方法原代培养Wistar大鼠皮质神经元,先后给予不同剂量的DHA(20、50和100μmol/L)及Aβ25-35(25μmol/L),用CCK-8比色法观察神经元存活率,用激光扫描共聚焦显微镜观察细胞内游离钙离子浓度。结果 1)与对照组相比,Aβ25-35使细胞存活率明显下降(31%±6%,P<0.05);使细胞内游离钙离子浓度明显升高(249%±12%,P<0.05);2)孵育DHA可降低Aβ25-35引起的神经元存活率明显下降及细胞内游离钙离子浓度升高。结论 Aβ致细胞内钙超载是Aβ产生神经毒作用的一个方面,而DHA可部分拮抗Aβ25-35的神经毒作用。Objective To observe the protection of Ducosahexaenoic acid （DHA）on primary culture cortical neu- rons exposed to amyloid beta protein 25-35. Methods Newborn Wistar rat cortical neurons were primarily cul- tured, then treated with DHA of different dose（20,50,100 μmol/L） and aggregated Aβ25-35 （25μmol/L）. CCK-8 （Cell Counting Kit-8 ）staining was used to detect the survival rate of cortical neurons, and LSCM （laser-scanning confocal imaging system）was used to detect the changes of intracellular free calcium concentration in neurons la- beled with the fluorescent dye Fluo-3/AM. Results 1 ） Compared with control group, the survival rate of cortical neurons was decreased in Aβ group （31%± 6% ,P 〈 0.05 ）, intracellular free calcium concentration in Aβ group was elevated（249% ± 12% ,P 〈0. 05 ） ; 2） incubation with DHA attenuated the decrease in the survivat rate of cor- tical neurons and the increase in intracellular free calcium concentration induced by amyloid beta protein 25-35. Conclusions 1 ） Calcium overloading induced by amyloid beta protein 25-35 might be the main neurotoxicity effect ; 2） DHA could partly decrease calcium overloading induced by amyloid beta protein 25-35, which might be the important mechanism of DHA attenuating the neurotoxicity effect.

关 键 词：二十二碳六烯酸(DHA) β淀粉样蛋白25—35(Aβ25-35) 钙离子(Ca2+) 皮质神经元 

分 类 号：R749.16[医药卫生—神经病学与精神病学]