ER-α36对胃癌SGC7901细胞在裸鼠体内生长的影响  

ER-α36 promotes the growth of SGC-7901 cells in nude mice

作　　者：王绪明[1] 刘晶晶[1] 邓昊[2] 陈莹[2] 刘丽江[2] 

机构地区：[1]武汉大学基础医学院,湖北省武汉市43007 [2]江汉大学医学院病理学与病理生理学教研室,湖北省武汉市430056

出　　处：《世界华人消化杂志》2011年第28期2919-2924,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30870981;湖北省卫生厅科研基金资助项目;No.NX200727~~

摘　　要：目的:观察ER-α36分子对人胃癌细胞SGC7901在裸鼠体内生长的影响.方法:利用慢病毒转染技术构建稳定高表达和低表达ERα-36分子的重组人胃癌SGC7901细胞株.实验分为空白对照组(SGC7901-Control)、ER-α36低表达组(SGC7901-Low36)和ER-α36高表达组(SGC7901-High36),每组各3只雄性裸鼠.将上述细胞(5×106个/mL)接种裸鼠皮下,建立裸鼠荷瘤模型,连续观测30d.采用瘤结节体积测量和称质量、瘤组织HE染色及免疫组织化学法检测Ki67指数和E-cadherin蛋白表达等方法,鉴定各组细胞生长的情况.结果:全组实验动物均出现移植瘤.第16天开始,SGC7901-High36组裸鼠肿瘤的体积>SGC7901-Control组裸鼠肿瘤的体积>SGC7901-Low36组裸鼠肿瘤的体积,两两之间有显著性差异(P<0.05).第30天,SGC7901-High36组肿瘤的质量(2.58g±0.014g),明显大于SGC7901-Control组(1.32g±0.0245g)和SGC7901-Low36组(0.471g±0.021g),两两之间有显著性差异(P<0.05).SGC7901-High36组肿瘤细胞的核分裂数(42.33±6.33),明显高于SGC7901-Control组(28.5±0.35)和SGC7901-Low36组(12.5±2.5),两两之间有显著性差异(P<0.05).ki67阳性细胞计数,SGC7901-High36组(86.35±5.23),明显高于SGC7901-Control组(65.44±4.56)和SGC7901-Low36组(18.25±2.56),两两之间有显著性差异(P<0.05).SGC7901-High36组肿瘤细胞几乎不表达E-cadherin蛋白,明显低于SGC7901-Control组和SGC7901-Low36组.结论:ER-α36分子在胃癌细胞的生长与增殖中具有重要作用,并可能通过靶向肿瘤细胞的粘附分子而促进肿瘤的侵袭与转移.AIM：To observe the effect of ER-α36 on thegrowth of SGC-7901 cells in nude mice.METHODS：Utilizing lentivirus technology,wedeveloped SGC7901 cell lines stably expressingER-α36 siRNA vector（SGC7901-Low36） andER-α36 expression vector（SGC7901-High36）.Unmanipulated SGC7901 cells were used as controls（SGC7901-Control）.These cells were subcutaneously injected into the nude mice to formSGC7901 transplantable tumors.The size andweight of the tumors were measured.Nucleardivision was observed after HE staining,and theexpression of Ki67 and E-cadherin was detectedby immunohistochemistry.RESULTS：Transplantable tumors formed in all nude mice.From day 16 to day 30,tumor size was highest in the SGC7901-High36 group,followed by the SGC7901-Control group,and the SGC7901-Low36 group had the least tumor size.There were significant differences（all P 0.05） in tumor size between any two groups.On day 30,tumor weight was signif icantly higher in the SGC7901-High36 group than in the SGC7901Control and SGC7901-Low36 groups（2.58 g ± 0.014 g vs 1.32 g ± 0.0245 g,0.471 g ± 0.021 g;both P 0.05）.The number of nuclear division phases was significantly higher in the SGC7901High36 group than in the SGC7901-Control and SGC7901-Low36 groups（42.33 ± 6.33 vs 28.5 ± 0.35,12.5 ± 2.5;both P 0.05）.The expression of Ki67 was significantly higher in the SGC7901High36 group than in the SGC7901-Control and SGC7901-Low36 groups（86.35 ± 5.23 vs 65.44 ± 4.56,18.25 ± 2.56;both P 0.05）.The expression of E-cadherin in tumors in the SGC7901-High36 group was hardly seen,signif icantly lower than that in the SGC7901-Control and SGC7901High36 groups.CONCLUSION：ER-α36 may play an important role in gastric cancer cell growth and proliferation.ER-α36 may target tumor cells through adhesion molecules to promote tumor invasion and metastasis.

关 键 词：雌激素受体-α36 胃癌 SGC7901 

分 类 号：R735.2[医药卫生—肿瘤]