核PTEN和Survivin蛋白在胃癌组织芯片中的表达及意义  

Signif icance of expression of nuclear PTEN and survivin in gastric cancer

作　　者：王晓茹[1] 李小宁[1] 白光辉[2] 郭云娣[1] 

机构地区：[1]苏州卫生职业技术学院苏州检验医学生物技术重点实验室,江苏省苏州市215009 [2]苏州市立医院,江苏省苏州市215008

出　　处：《世界华人消化杂志》2011年第28期2974-2978,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨核PTEN和Survivin蛋白在胃癌组织芯片中的表达、临床病理学特征及其意义.方法:在116例胃癌35例正常胃黏膜组织芯片上,应用免疫组织化学En Vision法检测核PTEN和Survivin表达水平,分析核PTEN和Survivin在胃癌的表达与患者淋巴结转移状态、Lauren's分型等的关系及其相互关系.结果:核PTEN和Survivin在正常胃黏膜组织中表达率为100%,核PTEN在胃癌组织中的阳性表达率为51.7%(60/116):Survivin的阳性表达率为44.0%(51/116),核PTEN和Survivin与患者淋巴结转移状态相关(P<0.05),同时,核PTEN还和肿瘤分化程度、Lauren's分型密切相关(P<0.05).核PTEN和Survivin二者之间呈正相关(r=0.088,P=0.001).结论:胃癌组织中存在核PTEN和Survivin低表达:同时检测胃癌组织中的核PTEN和Survivin的表达对判断肿瘤的恶性程度和估计预后有一定意义.AIM：To investigate the expression of nuclear PTEN and survivin in gastric cancer and to evaluate their clinicopathological significance.METHODS：The expression of nuclear PTEN and survivin was measured in 116 cases of gastric cancer and 35 cases of normal gastric mucosa tissue on tissue array sections by EnVision immunohistochemical method.The relationship of their expression with lymph node metastasis and Lauren＇s classification,as well as the correlation between nuclear PTEN and survivin expression were evaluated.RESULTS：The positive rates of nuclear PTEN and survivin in normal gastric mucosa were higher than those in gastric cancer（100% vs 57.1%;100% vs 44.0%）.The expression of nuclearPTEN and Survivin was significantly correlated with lymph node metastasis in gastric cancer（P 0.05）.Nuclear PTEN was signif icantly related to Lauren＇s classif ication and differentiation（both P 0.05）.The expression of nuclear PTEN had a significant correlation with that of survivin（r = 0.088,P = 0.001）.CONCLUSION：Nuclear PTEN and survivin are lowly expressed in gastric cancer.Nuclear PTEN and survivin may be good predictors of metastasis and prognosis of gastric cancer.

关 键 词：胃癌 第10号染色体同源缺失性磷酸酶-张力蛋白基因 SURVIVIN 组织芯片 

分 类 号：R735.2[医药卫生—肿瘤]