αvβ6-ERK直接通路在乌司他丁抑制结肠癌侵袭转移中的作用机制  

Role of αvβ6-ERK direct pathway in the inhibitive effects of ulinastatin on colon cancer

作　　者：王奔[1] 刘恩宇[1] 牛卫博[1] 彭程[1] 王健[1] 牛军[1] 

机构地区：[1]山东大学齐鲁医院肝胆外科,山东济南250012

出　　处：《中国现代普通外科进展》2011年第10期772-775,共4页Chinese Journal of Current Advances in General Surgery

基　　金：天普研究基金(01200901)

摘　　要：目的:探讨αvβ6-ERK直接通路在乌司他丁(UTI)抑制结肠癌侵袭转移中的作用。方法:100例结肠癌患者随机分为对照组和治疗组,ELISA检测血清MMP-9/2水平,免疫组织化学检测结肠癌组织αvβ6表达;HT-29细胞按UTI不同浓度分组,Transwell小室检测细胞侵袭能力,明胶酶谱检测MMP-9/2水平,Wes tern Blot检测细胞αvβ6和ERK变化。结果 :UTI可降低结肠癌患者血清MMP-9/2水平及肿瘤组织αvβ6表达强度;UTI可抑制HT-29细胞侵袭能力及MMP-9/2分泌水平,并显著下调αvβ6表达和ERK磷酸化水平。结论:UTI可显著抑制结肠癌的侵袭浸润,αvβ6-ERK直接通路介导的MMP-9/2分泌可能在其中发挥重要作用。Objective： To investigate the role of αVβ6-ERK direct pathway in the inhibitive effects of ulinastatin（UTl） on colon cancer. Methods： 100 cases of colon cancer were randomly divided into control and treatment group. ELISA was used to detect MMP-9/2 levels in serum and immunohistochemistry to observe αVβ6 expression in cancer tissue; HT-29 cells were randomly di vided into different groups by UT/concentration. Transwelt invasion assay was apptied to detect invasive ability and zymography to analyze MMP-9/2 levels, Western blot were used to detect αVβ6 expression and ERK changes, libesults： UTI reduced MMP-9/2 levels in serum and αVβ6 expression in tumor tissue. Meanwhile, UTI significantly inhibited invasive ability and MMP-9/2 secretion of HT-29 cells. In addition, Western blot demonstrated that αVβ6 and p-ERK expression decreased with increasing doses of UTI. Conclusion： UTI can significantly inhibit invasion of colon cancer, and αVβ6-ERK mediated MMP-9/2 secretion might play a key role in this process.

关 键 词：结肠癌 乌司他丁 整合素 αvβ6 ERK 

分 类 号：R735.35[医药卫生—肿瘤]