组织工程皮肤的构建及组织形态学观察  

Construction and histomorphological observation of tissue-engineered skins

作　　者：董丽[1] 王旭昇[1] 马绍英[1] 张乃丽[1] 周沫[1] 赵亚平[1] 李宝兴[1] 

机构地区：[1]中国辐射防护研究院,山西省太原市030006

出　　处：《中国组织工程研究与临床康复》2011年第41期7631-7634,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:应用于临床的组织工程皮肤具有血管化速度慢、力学强度差以及无法永久性保留等局限,因此,需要对相关技术环节进行改进以制备一种合适的永久性皮肤替代物。目的:建立一种构建有活性的双层组织工程皮肤的方法,并对其组织形态学进行观察。方法:以Ⅳ型胶原和成纤维细胞联合修饰的同种脱细胞真皮基质为支架,与表皮干细胞复合后依次经浸没式培养和气液分离界面培养构建组织工程皮肤,利用光镜和扫描电镜对其组织学特点进行观察和分析。结果与结论:以表皮干细胞和同种脱细胞真皮基质制备的组织工程皮肤具有表、真皮双层结构,其中表皮由多层不同分化程度的表皮细胞组成,真皮为天然三维孔隙结构,胶原纤维完整,且表皮层与真皮层紧密连接,形成整体结构,可满足全层皮肤替代物的基本组织学要求。BACKGROUND：Tissue-engineered skins are the earliest tissue-engineered products applied to the patients.However,some important properties including rapid revascularization,mechanical strength and permanent substitution are not perfect and remain to be improved.Therefore,relevant technical steps are expected to be taken to develop an ideal permanent skin substitute.OBJECTIVE：To construct tissue-engineered skins and observe the histological structure.METHODS：Allogenic acellular dermal matrix（ADM） as the dermal scaffold,pretreated with collagen Ⅳ and fibroblasts,were co-cultured with epidermal stem cells to construct tissue-engineered skins in vitro.Two steps during the co-culture were performed including submerging culture and air-liquid interface culture.Thereafter,the histological structure was observed by light microscopy and scanning electron microscopy.RESULTS AND CONCLUSION：The constructed tissue-engineered skins were made of two layers including the epidermis and dermis,the former consisted of multi-layer of epidermal cells in different differentiation conditions and the latter was the integral three-dimensional scaffold with intact collagen fibers.Tight connection could be seen between the epidermis and the dermis to form the integrated ＂skin＂.So,in terms of histological structure,the constructed tissue-engineered skin can reach the histological requirements for the full-thick skin substitutes and can be further studied to be used as the replacement materials to repair skin defects in the clinic.

关 键 词：表皮干细胞 成纤维细胞 同种脱细胞真皮基质 组织工程皮肤 扫描电镜 

分 类 号：R318[医药卫生—生物医学工程]