SPIO标记脂肪干细胞移植退变椎间盘内的MRI活体示踪  

In vivo tracking superparamagnetic iron oxide labeled adipose derived stem cells to repair rabbit degenerated intervertebral disc

作　　者：蒋新华[1] 陈建宇[2] 蔡兆熙[2] 张娅[2] 刘珍珍[2] 谢传淼[1] 伍尧泮[1] 吴沛宏[1] 

机构地区：[1]华南肿瘤学国家重点实验室中山大学肿瘤防治中心影像与微创介入中心,广东省广州市510060 [2]中山大学孙逸仙纪念医院放射科,广东省广州市510120

出　　处：《中国组织工程研究与临床康复》2011年第40期7515-7519,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广东省科技计划项目资助(2008B030301318);Gd-MSCs移植修复退变椎间盘及MRI活体示踪的实验研究~~

摘　　要：背景:国内外动物实验多是荧光标记骨髓间充质干细胞的移植,以SPIO标记脂肪干细胞移植后活体示踪对退变椎间盘修复作用的研究较少。目的:活体监测SPIO标记的脂肪干细胞在退变椎间盘内的存活、迁移和转归,以及脂肪干细胞对退变椎间盘的修复及延缓退变作用。方法:新西兰大白兔20只,兔椎间盘被分为4组,即正常对照组(L1/2),脂肪干细胞组(L2/3),PBS组(L3/4),SPIO-脂肪干细胞组(L4/5)。透视引导下用18G穿刺制作退变模型后2周行SPIO标记的脂肪干细胞移植。结果与结论:SPIO-脂肪干细胞移植后即刻T2WI/FFE序列上可见椎间盘内明显低信号,8周后仍可检测到低信号。脂肪干细胞移植组与同时间点PBS组比较,椎间盘退变程度轻。提示SPIO-脂肪干细胞移植至椎间盘后,可通过MRI进行监测;脂肪干细胞椎间盘内移植有助于修复退变椎间盘和延缓椎间盘退变。BACKGROUND： Numerous studies have demonstrated fluorescent labeled bone marrow mesenchymal stem cells transplantation, but few reports have addressed in vivo tracking of superparamagnetic iron oxide （SPIO） labeled adipose derived stem cells （ADSCs） and its contribution to repair degenerated intervertebral disc. OBJECTIVE： To In vivo track transplanted SPIO labeled ADSCs survival and migration by MRI, and to investigate the contribution of ADSCs to repair degenerative intervertebral disc. METHODS： Twenty rabbits were divided into four groups： normal group （L1/2）, ADSCs group （L2/3）, PBS group （L3/4）, SPIO-labeled ADSCs group （L4/5）. X-ray photograph, MR scanning and histopathological examination were done in time interval after SPIO labeled ADSC was transplanted. RESULTS AND CONCLUSION： SPIO labeled ADSCs transplanted intervertebral disc showed low signal intensity on T2WI/FFE, and low signal intensity remained on T2WI/FFE even after 8 weeks in transplanted SPIO labeled ADSC intervertebral disc. Compared with the PBS group, ADSCs group showed a lesser extent degeneration. SPIO labeled ADSCs transplanted intervertebral disc can be tracked by MRI; ADSCs transplantation is effective in regeneration of degenerated intervertebral disc.

关 键 词：椎间盘退变 脂肪干细胞 超顺磁性氧化铁 细胞移植 MRI 

分 类 号：R394.2[医药卫生—医学遗传学]