三维培养条件下胚胎鼠唾液腺上皮细胞的自组装  

Self-organization and branching morphogenesis of primary salivary epithelial cells under three-dimensional culture

作　　者：王军胜[1] 黄桂林[2] 张霓霓[2] 姚礼[2] 唐岳鹏[2] 

机构地区：[1]西安医学院附属医院口腔科,陕西省西安市710077 [2]遵义医学院附属口腔医院口腔颌面外科,贵州省遵义市563003

出　　处：《中国组织工程研究与临床康复》2011年第40期7549-7553,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：贵州省优秀科技教育人才省长资金[黔省专合字(2008)113号];贵州省教育厅基金[(2006)354号]~~

摘　　要：背景:对唾液腺胚胎细胞在体外自组装及形态发生过程的理解,将有利于在体外构建唾液腺器官样组织的研究。目的:探讨三维培养条件下SD大鼠胚胎鼠下颌下腺上皮细胞的自组装机制。方法:将胚胎期15d鼠的下颌下腺上皮细胞分为实验组(加入抗E-cadherin及抗β-catenin)和对照组(常规培养),在Matrigel中三维培养。结果与结论:获得的胚胎鼠下颌下腺上皮细胞均表达CK19。实验组细胞未见分枝状及腺泡样结构出现;E-cadherin和β-catenin荧光表达弱。对照组细胞在12d出现上述结构;胞浆中E-cadherin、β-catenin荧光表达强表达。MOD值为实验组<对照组(P<0.01)。提示E-cadherin,β-catenin在细胞的自组装过程中有重要作用。BACKGROUND： To understand self-assembly and branching of primary salivary epithelial cells will provide insights into reconstructing artifical salivary gland orgnoid. OBJECTIVE： To investigate the machanism of self-organization and branching mophogenesis of primary submandibular epithelial cells under three-dimensional condition. METHODS： The embryonic day 15 （E 15） mouse submandibular gland epithelium were inoculated in matrigel for 3-dimantional culture with/without （Group A/Group B） anti-E-cadherin and and anti-β-catenin. The contructs of these cells aggregation were observed under inverted microscope at days 1, 6, 12. The contructs were observed with cytokeratin 19 immunohistochemistry staining, and identified with Cadherin and β-catenin immunostaining and confocal microscopy. MAIN OUTCOME MEASURES： The constructs were observed under inverted microscope and analyzed by antibody of cytokeratin 19 with immunohistochemistry, and identified with cadherin and β-catenin immunostaining and confocal microscopy. In Group A, branching morphogenesis and acinar-like structure were not observed. In Group B, these contructs could be formed at day 12. In Group A, weak green fruorescence of E-cadherin and β-catenin expressed weakly. In Group B, they expressed strongly, and in the cytoplasm. MOD was lower in Group A than Group B （P 0.01）. E-cadherin and β-catenin are two important factors during the embryonic submandibular salivary gland epithelium self-organization

关 键 词：下颌下腺上皮细胞 胚胎鼠 三维培养 自组装 E-CADHERIN Β-CATENIN 

分 类 号：R394.2[医药卫生—医学遗传学]