引导骨组织再生技术结合碱性成纤维细胞生长因子和骨保护素修复犬牙槽骨缺损  

Osteoprotegerin and basic fibroblast growth factor combination enhances the restoration of alveolar bone defects in dogs

作　　者：曹寅[1] 何世海[2] 陈乔尔[2,3] 

机构地区：[1]常州市口腔医院牙周科,江苏省常州市213003 [2]安徽医科大学口腔医学院,安徽省合肥市230032 [3]安徽省口腔疾病研究省级实验室,安徽省合肥市230032

出　　处：《中国组织工程研究与临床康复》2011年第42期7801-7804,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：常州市社会发展科技计划资金(CS20092023)~~

摘　　要：背景:多种生长因子能有效促进牙周组织再生。目的:观察骨保护素和碱性成纤维细胞生长因子对牙槽骨骨缺损的修复作用。方法:在4只杂种犬下颌两侧的第3,4前磨牙根分叉区造牙槽骨缺损,每只犬的一侧为实验组,另一侧为对照组。实验组置胶原膜并于缺损部位牙龈注射携带骨保护素和碱性成纤维细胞生长因子基因的重组腺病毒,对照组仅注射重组腺病毒或不做处理。结果与结论:缺损修复4周后,实验组和对照组在X射线和组织学等方面差异不显著。12周后,实验组X射线可见骨缺损区新生骨量明显增加,组织学见新生牙槽骨已充满根分叉区,骨小梁致密;对照组X射线可见骨缺损区新生骨量增加,但未达到根分叉顶,组织学见骨缺损区有大量新生牙槽骨但未充满根分叉区。说明引导骨组织再生技术结合骨保护素和碱性成纤维细胞生长因子可促进犬牙槽骨缺损的修复。BACKGROUND： A variety of growth factors are effective in promoting periodontal tissue regeneration. OBJECTIVE： To explore the restoration of alveolar bone defects with recombinant adenovirus encoding osteoprotegerin and basic fibroblast growth factor. METHODS： Class Ⅲ furcation defects were created in the mandibular premolars. The osteoprotegerin and basic fibroblast growth factor combination with adenovirus was covered on the defects in the experimental group, while in the control group only with adenovirus or nothing. RESULTS AND CONCLUSION： Four weeks after operation, the difference between the experimental group and the control group was indistinctive. Twelve weeks after operation, the new bone filled almost the entire circumference of the furcation in the experimental group, while the quantity of the new bone was less in the control group. Combination of osteoprotegerin and basic fibroblast growth factor could promote alveolar bone regeneration.

关 键 词：骨保护素 碱性成纤维细胞生长因子 引导骨组织再生 基因工程 牙组织工程 

分 类 号：R318[医药卫生—生物医学工程]