3D自组装肽纳米纤维支架对胰岛细胞分泌功能的影响  

A three-dimensional self-assembling peptide nanofiber hydrogel scaffold influences the secretion function of islet cells in vitro

作　　者：黄任平[1] 宋纯[1] 张增岭[1] 范杰后[1] 石于波[1] 宋春芳[1] 

机构地区：[1]哈尔滨医科大学附属第一医院,卫生部细胞移植重点实验室及普通外科,黑龙江省哈尔滨市150001

出　　处：《中国组织工程研究与临床康复》2011年第42期7863-7867,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30570931)~~

摘　　要：背景:3D自组装肽纳米纤维支架能很好模拟体内微环境,给予细胞必要的结构模式,促进细胞外基质的正确组成及细胞的生长,改善细胞功能。目的:体外观察3D自组装肽纳米纤维水凝胶支架对胰岛细胞分泌功能的影响。方法:将3D自组装肽纳米纤维水凝胶支架与成年大鼠胰岛细胞共培养,AO-PI荧光染色法检测胰岛细胞的活性及生存率;放射免疫法测定胰岛细胞的分泌功能;扫描电镜观察胰岛细胞包裹在3D纳米支架中成三维立体的生长状态。结果与结论:在3D纳米支架培养环境中胰岛细胞纯度≥80%;3D纳米支架组胰岛生存率及胰岛细胞分泌功能明显高于无支架组(2D培养组)(P<0.05);扫描电镜显示自组装肽纳米纤维支架形成了具有几何形状的纳米级薄层,将胰岛细胞包裹在3D纳米支架中,胰岛细胞成三维立体生长。表明3D自组装肽纳米纤维支架可为胰岛细胞体外生存提供3D培养环境,改善胰岛细胞的活性、分泌功能及形态,延长胰岛细胞体外生存期。BACKGROUND： Three-dimensional self-assembling peptide nanofiber hydrogel scaffold can simulate the in vivo microenvironment and provide the structural model for cells, which promotes the right composition of extracellular matrix and cell growth, as well as improve the cell functions. OBJECTIVE： To observe the influence of three-dimensional self-assembling peptide nanofiber hydrogel scaffold on the secretion function of islets in vitro. METHODS： We co-cultured the three-dimensional nanofiber hydrogel scaffold and islet cells from adult Wistar rats. The AO-PI fluorescent staining was used to detect the viability and survival rates of islet cells; radioimmunoassay to determine the secretion function of islet cells; the scanning electron microscopy to observe three-dimensional growth condition of islet cells encapsulated in the self-assembling peptide nanofiber hydrogel scaffold. RESULTS AND CONCLUSION： The purity of the islet cells in the peptide scaffold was 80%. The survival rates and secretion function of the islet cells in the three-dimensional nanofiber scaffold were significantly higher than those in the two-dimensional culture environment （P 0.05）. By the scanning electron microscopy, it was observed that the self-assembling peptide nanofiber hydrogel scaffold formed a nano-scale folium with a geometric form and the islet cells encapsulated in this scaffold were in three-dimensional growth. The results demonstrated that the three-dimensional self-assembling peptide nanofiber hydrogel scaffold provides a favorable microenvironment for the islet cells to grow in vitro and also can improve the viability, the secretion function and morphology, as well as prolong the survival time of islet cells in vitro.

关 键 词：纳米纤维支架 胰岛细胞 扫描电镜 分泌功能 生物材料与纳米技术 

分 类 号：R318[医药卫生—生物医学工程]