细胞周期素依赖蛋白激酶-5抑制剂Roscovitine对瑞芬太尼痛觉过敏大鼠痛行为的影响  

Effects of cyclin dependent kinase 5 inhibitor Roscovitine on remifentanil-induced hyperalgesia

作　　者：刘晓杰[1] 顾小萍[2] 张威[2] 张兢[2] 马正良[2] 

机构地区：[1]南京医科大学附属鼓楼医院麻醉科,南京210008 [2]南京市鼓楼医院麻醉科

出　　处：《中华行为医学与脑科学杂志》2011年第11期961-963,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（30872439/c16060202）;六大人才高峰项目（WSW-004）;江苏省自然基金项目（BK2010105）;江苏省卫生厅课题（H200943）;南京市卫生局重大课题（zkx08028）

摘　　要：目的探讨预先鞘内注射细胞周期素依赖蛋白激酶-5（Cdk5）抑制剂Roscovitine对瑞芬太尼痛觉过敏大鼠痛行为的影响。方法SD雄性大鼠45只，随机数字表法分成5组（n=9）：对照组（C）、切口痛组（I）、瑞芬太尼组（R）、Roscovitine组（ROS）、Roscovitine＋瑞芬太尼组（ROS＋R）。ROS，ROS＋R组术前30min鞘内给予Roscovitine10μl（50μg），余组给予20％DMSO10μl。除C组外均制作切口痛模型；R和ROS＋R组切皮同时皮下泵注瑞芬太尼0．4ml（0．04mg／kg）30min。于术前24h、术后2h、6h、24h、48h检测大鼠术侧足底机械缩足阈值（paw withdrawal mechanical threshold，PWMT）和热缩足潜伏期（paw withdrawal thermal latency，PWTL）。结果与C组相比，Ⅰ组术后PWMT、PWTL明显降低（P〈0．01）。与Ⅰ组相比，R组术后PWMT，PWTL明显降低（P〈0．01）；而ROS组术后PWTL明显升高[2h：（20．26±1．33）S，（17．97±0．47）S；48h：（22．15±0．60）s，（19．89±1．27）s]（P〈0．05）。ROS＋R与R组相比，术后2hPWTL[（19．13±1．72）S，（14．41±2．30）s]及PWMT[（10．4±1．95）g，（6．38±0．91）g]开始升高，分别持续至48h[（19．24±2．80）S，（14．87±1．95）s]和24h[（8．88±1．41）g，（6．83±0．80）g]（P〈0．05）。结论Roscovitine能减轻大鼠切口引起的热痛敏，并能缓解瑞芬太尼诱发的切口痛大鼠痛觉过敏。Objective To study the effects of intrathecal injection of cyclin dependent kinase 5 inhibitor Roscovitine on the hyperalgesia induced by remifentanil in a rat model of incisional pain. Methods Forty-five SD rats were randomly divided into 5 groups （ n = 9 in each group） ： control group （ C ）, incisional pain group （ I）, Roscovitine group（ ROS）, remifentanil group （R） and Roscovitine ＋ remifentanil group （ROS ＋ R）. Roscovitine （50μg/10μl） was injected intrathecally at 30 rain before plantar incision in groups ROS and ROS ＋ R, other groups were injected with 20% DMSO（10μl）. All groups except for C group needed to be made the model of inci- sional pain. In group R and ROS ＋ R , remifentanil （0.04 mg/kg） was infused subcutaneously with a pump for 30 min at the moment of surgical incision. The paw withdrawal mechanical threshold（PWMT） and paw withdrawal thermal latency（PWTL） were used to evaluate the behavioral changes and measured at 24 h before incision and at 2 h,6 h,24 h and 48 h after incision. Results Compared with group C, the PWMT and PWTL of group I were significantly decreased after incision （P 〈 0.01 ）. Compared with group I, the PWMT and PWTL of group R were significantly decreased after incision （P 〈 0.01 ） ; however, the PWTL was significantly increased （ 2b ： （ 20.26 ± 1. 33 ） s, （ 17.97 ± 0.47） s ; 48h ： （ 22.15 ± 0.60 ） s, （ 19.89 ±1.27 ） s）, P 〈 0.05 ） in ROS group. Compared with group R, at 2h after incision,the significant increase of PWTL（ （19.13± 1.72）s, （14.41 ± 2.30）s） and PWMT （ （ 10.4 ± 1.95） g, （6.38 ± 0.91 ） g） were observed, then lasted up to 48h （ （ 19.24 ± 2.8 ） s, （ 14.87± 1.95 ） s） and24h（（8.88±1.41）g,（6.83±0.80）g） respectively in group ROS＋R（P〈0.05）.Conclusion In a rat model of postoperative pain, Roseovitine could reduce the thermal hyperalgesia purely induced by incision, and also could prevent the development of hypera

关 键 词：细胞周期素依赖蛋白激酶-5 ROSCOVITINE 瑞芬太尼 切口痛 痛觉过敏 NMDA 受体 

分 类 号：R614[医药卫生—麻醉学]