咖啡因对链尿霉素诱发大鼠β-细胞损伤的保护作用  

Protective effect of caffeine on streptozotocin-induced beta-cell damage in rats

作　　者：梁艳[1] 陈充抒[1] 梁莉[2] 

机构地区：[1]武警总医院药剂科,北京100039 [2]武警总医院超声科,北京100039

出　　处：《武警医学》2011年第11期932-934,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨咖啡因对链尿霉素(streptozotocin,STZ)诱导大鼠β-细胞损伤的影响。方法 Wistar大鼠皮下注射生理盐水(对照组)或咖啡因(治疗组)。15 min后,大鼠再皮下给柠檬酸缓冲或STZ(65 mg/kg)。注射后第3天对大鼠进行口服葡萄糖耐量试验(OGTT)。OGTT后第3天分离胰腺测定其中胰岛素水平。结果 STZ处理的大鼠,与对照组比较,在OGTT测试所有时间点都明显增加血浆葡萄糖水平。预先给咖啡因可明显减低ST2的升高血糖作用。STZ处理还可明显减少血浆胰岛素的浓度,但预先给咖啡因任何剂量不能逆转血浆胰岛素降低的作用。与对照组比较,STZ处理可明显降低胰腺中胰岛素水平。此作用也能被咖啡因所逆转。结论此研究结果表明咖啡因对STZ损伤大鼠胰岛β-细胞,具有一定的保护效应。但要了解咖啡因对2型糖尿病患者β-细胞是否有保护效果尚需进一步研究。Objective To investigate the effect of caffeine on pancreatic beta - cell damage in rats using the diabetogenic agent streptozotocin （STZ）. Methods Wistar rats were given intraperitoneal injections of saline or caffeine（ 10,50 or 100 mg/kg）. After 15 min, the rats were injected with a citrate buffer or 65 mg/kg STZ. Three days after injection, an oral glucose tolerance test（ OG- TF） was performed on the rats. Furthermore, three days after the OGTT, the pancreas was isolated and homogenized, followed by determination of the insulin content. Results STZ treatment significantly increased the plasma glucose level, compared with the control throughout the OGTT ,which was significantly reduced by caffeine pretreatment at each dose. STZ treatment significantly decreased the plasma insulin level, which, however, was not recovered by caffeine pretreatment. Pancreatic insulin content was significantly reduced by STZ treatment, compared with the control, which was significangly recovered by caffeine pretreatment, Conclusions Caffeine protects pancreatic beta - cells against STZ toxicity. Further investigation is required to understand the protective effect of caffeine against beta - cell destruction in type 2 diabetes mellitus.

关 键 词：大鼠 链尿霉素 2型糖尿病 咖啡因 

分 类 号：R917.1[医药卫生—药物分析学]