大肠癌E2F-1、CyclinD1的表达及其意义  

Expression of E2F-1 and cyclinD1 in colorectal adenocarcinoma and its significance

作　　者：王蕾[1] 何妍 李希芳 刘岩[4] 时志民[1] 刘惠民[1] 

机构地区：[1]河北工程大学医学院病理学教研室,邯郸056029 [2]河北武安中医院,056300 [3]河北省邯郸市第五医院,056000 [4]河北省邯郸市第二医院,056010

出　　处：《武警医学》2011年第11期935-937,942,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：邯郸市科学技术局资助项目(0920108055-3)

摘　　要：目的探讨E2F-1和cyclinD1在大肠腺癌组织中的表达及其意义。方法采用脱氧核糖核酸末端转移酶介导的TUNEL法缺口末端标记和免疫组化SP法检测大肠腺癌78例、正常大肠黏膜20例、大肠息肉30例,以及大肠腺瘤细胞30例的原位凋亡(Al)及E2F-1和cyclin D1的表达情况。结果 E2F-1和cyclin D1在大肠腺癌中的阳性率显著高于正常大肠黏膜、大肠息肉以及大肠腺瘤(P<0.05)。E2F-1表达与大肠腺癌的病理分化程度、淋巴结转移和临床分期具有相关性(P<0.05)。从正常大肠黏膜、大肠息肉、大肠腺瘤到大肠腺癌细胞凋亡呈梯度降低。结论 E2F-1和cyclinD1的表达与大肠腺癌发生关系密切,是大肠腺癌发生、发展的重要生物学标记物。Objective To study the expression of E2F - 1 and cyclinD1 in human colorectal adenocarcinoma and its significance. Methods Terminal deoxynucleotidyl transferase - mediated nick end labeling TUNEL method and immunohistochemical SP method were used to detect 78 cases of eolorectal adenocarcinoma, 20 cases of normal colorectal mucosa, 30 cases of colorectal polyps, and 30 cases of colorectal adenoma cells in situ withered death （ AI）, and E2F - 1 and cyclinD1 expression. Results The positive expression rate of E2F - 1 and eyclinD1 in esophageal carcinoma was much higher than that in colorectal mucosa, colorectal polyps, and colorectal adenoma （ P 〈 0.05 ）. The expression of E2F - 1 was closely correlated with the differentiation, TNM and lymph node metastasis （ P 〈 0. 05 ）. Conclusions E2F - 1 and eyclinD1 are closely related to the occurrence of colorectal adenoearcinoma. Both of them are important biological markers in eolorectal adenocareinoma occurrence and development.

关 键 词：大肠腺癌 E2F-1 CYCLIND1 细胞凋亡 

分 类 号：R735.34[医药卫生—肿瘤]