Apolipoprotein B100 analysis in microchip with electrochemical detection  

作　　者：Cheng Cheng Liu Yun Liu Hui Xiang Wang Yan Bo Qi Peng Yuan Yang Bao Hong Liu 

机构地区：[1]Department of Chemistry and Institute of Biomedical Science, Fudan University, Shanghai 200433, China

出　　处：《Chinese Chemical Letters》2011年第11期1343-1346,共4页中国化学快报（英文版）

基　　金：supported by 973 Program(No2007CB714506);NSFC(Nos20925517,21005019);STCSM(Nos 10XD1406000,09JC 1402600);Shanghai Leading Academic Discipline Project(NoB109)

摘　　要：Apolipoprotein B100（apoB-100） is a major protein of the cholesterol-rich low-density lipoprotein（LDL） and reflects a better assessment of total atherogenic burden to the vascular system than LDL.In this work,a simple and sensitive method has been developed to determine picoliter apoB-100s using the PMMA microfluidic chip coupled with electrochemical detection system. This method performs very well with a detectable linear range of 1-800 pg/mL and a detection limit of 1 pg/mL.A real serum sample has further been detected by this microchip-based biosensor.The results show that this kind of method is practicable and has the potential application in clinical analysis and diagnosis.Apolipoprotein B100（apoB-100） is a major protein of the cholesterol-rich low-density lipoprotein（LDL） and reflects a better assessment of total atherogenic burden to the vascular system than LDL.In this work,a simple and sensitive method has been developed to determine picoliter apoB-100s using the PMMA microfluidic chip coupled with electrochemical detection system. This method performs very well with a detectable linear range of 1-800 pg/mL and a detection limit of 1 pg/mL.A real serum sample has further been detected by this microchip-based biosensor.The results show that this kind of method is practicable and has the potential application in clinical analysis and diagnosis.

关 键 词：MICROCHIP ApoB-100 IMMUNOASSAY Electrochemical detection 

分 类 号：Q513.5[生物学—生物化学] O657.1[理学—分析化学]