Molecularly imprinted macroporous monolithic materials for protein recognition  

作　　者：Qi Liang Deng Yan Li Li Li Hua Zhang Yu Kui Zhang 

机构地区：[1]Department of Science, Tianjin University of Science and Technology, Tianjin 300222, China [2]National Chromatograp＇hic R. ＆ A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

出　　处：《Chinese Chemical Letters》2011年第11期1351-1354,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos21075089 and 20605024);Talents Fund of Tianjin University of Science and Technology(No20090424)

摘　　要：Synthetic materials that can specifically recognize proteins will find wide application in many fields.In this report,bovine serum albumin was chosen as the template protein.Acrylamide and N,N＇-methylenebisacrylamide were employed as the functional and cross-linker monomers,respectively.Molecularly imprinted macroporous monolithic materials that can preferentially bind the template protein in an aqueous environment were prepared by combination of molecular imprinting technique and freezing/thawing preparation method.The resulted imprinted macroporous monolithic columns were evaluated by utilizing as stationary phase in high performance liquid chromatography and solid-phase extraction materials.The experimental results indicated that the imprinted macroporous monolithic column exhibited good recognition for template protein,as compared with the control protein（hemoglobin）,whereas the non-imprinted polymer（prepared under the same conditions except without addition template protein） had no selective properties.Synthetic materials that can specifically recognize proteins will find wide application in many fields.In this report,bovine serum albumin was chosen as the template protein.Acrylamide and N,N＇-methylenebisacrylamide were employed as the functional and cross-linker monomers,respectively.Molecularly imprinted macroporous monolithic materials that can preferentially bind the template protein in an aqueous environment were prepared by combination of molecular imprinting technique and freezing/thawing preparation method.The resulted imprinted macroporous monolithic columns were evaluated by utilizing as stationary phase in high performance liquid chromatography and solid-phase extraction materials.The experimental results indicated that the imprinted macroporous monolithic column exhibited good recognition for template protein,as compared with the control protein（hemoglobin）,whereas the non-imprinted polymer（prepared under the same conditions except without addition template protein） had no selective properties.

关 键 词：Molecularly imprinted polymers PROTEIN Monolithic column High performance liquid chromatography 

分 类 号：Q51[生物学—生物化学] O641.3[理学—物理化学]