DNA修复基因hOGG1多态性、HBV感染交互作用与肝细胞癌易感性的关系  

Interaction between the single-nucleotide polymorphism of DNA repair gene hOGG1 and HBV infection and its susceptibility to hepatocellular carcinoma

作　　者：纪龙[1] 曾小云[2] 李龙嫚[2] 仇小强[3] 陈似霞[4] 余红平[2] 

机构地区：[1]泰山医学院公共卫生学院,泰安271000 [2]广西医科大学公共卫生学院 [3]桂林医学院 [4]广西医科大学第一附属医院肝胆血管外科

出　　处：《卫生研究》2011年第6期705-708,共4页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30660162);广西研究生教育创新计划资助项目(No.2008105981004M189);泰山医学院2010年青年科学基金项目(No.2010ZRQN042)

摘　　要：目的探讨广西肝癌高发区人群DNA修复基因8-羟基鸟嘌呤糖苷酶1单核苷酸多态性与肝细胞癌易感性的关系,并分析与环境危险因素的交互作用。方法采用以医院为基础的病例-对照研究,对广西壮族自治区500例肝细胞癌和507例正常对照进行流行病学调查,并应用TaqMan MGB荧光定量PCR方法检测hOGG1-Ser326Cys位点基因型。结果病例组和对照组中hOGG1-326Cys突变等位基因分布频率为24.60%和10.75%(P<0.05);该位点3种基因型Ser/Ser、Ser/Cys和Cys/Cys在病例组和对照组中分布频率分别为71.40%、8.00%、20.60%和84.22%、10.06%、5.72%(P<0.05);与携带hOGG1基因326位点野生纯合子Ser/Ser基因型者比较,携带至少一个hOGG1-326Cys突变等位基因的个体患肝癌风险显著增加(OR=2.14,95%CI:1.57～2.91);交互作用分析结果表明hOGG1基因326位点多态性与慢性HBV感染之间在肝细胞癌发生中具有交互作用,相乘模型交互作用指数为0.61,相加模型交互作用指数为1.31。结论 hOGG1-326Cys突变等位基因是肝细胞癌发生的危险等位基因;携带至少一个hOGG1-326Cys突变等位基因的个体若同时暴露于HBV慢性感染,患肝癌的风险显著增加。Objective To explore the relationship between the single-nucleotide polymorphism of DNA repair gene hOGG1and the susceptibility to hepatocellular carcinoma（HCC）,and the interaction between hOGG1and environmental risk factors in a hepatocarcinoma high risk area.Method This hospital-based case control study was conducted in 500 HCC patients and 507 controls.The polymorphism of hOGG1-Ser326Cys was genotyped by a real time polymerase chain reaction-TaqMan MGB.Results The allele mutation frequencies of hOGG1-326Cys in the case group and control group were 24.60% and 10.75% respectively（P0.05）.The genotype frequencies of hOGG1 Ser/Ser,Ser/Cys,Cys/Cys were 71.40%,8.00% and 20.6% in the case group,and were 84.22%,10.06% and 5.72% in the control group respectively（P0.05）.Compared with the wild hOGG1-326Ser/Ser genotype,the mutant hOGG1allele increased the risk of HCC（OR=2.14,95%CI： 1.57-2.91）.The hOGG1-326Cys allele was the major determinant factor for the risk of HCC.The interaction of hOGG1-326Cys allele with chronic HBV infection（OR=38.15,95%CI： 18.90-61.71） tremendously increased the risk of HCC.The synergy index of multiplicative interaction is 0.61 and the synergy index of additive interaction is 1.31.Conclusion The hOGG1-326Cys allele may contribute to the risk of HCC.The synergistic interaction between hOGG1-326Cys allele and chronic HBV infection might modify the risk of HCC.Further studies with larger sample size are warranted to confirm these findings.

关 键 词：HOGG1 单核苷酸多态性 环境因素 交互作用 肝细胞癌 

分 类 号：R730.2[医药卫生—肿瘤] Q754[医药卫生—临床医学]