转录因子Pitx3和Nurr1在中脑多巴胺能神经元终末分化中的表达特点  

The characteristic of the transcription factors Pitx3 or Nurr1 expression in the terminal differentiation of mesencephalic dopaminergic neurons

作　　者：姜宇[1,2] 曾水林[1] 李涛[1] 李凤飞[1] 杨鎏[1] 刘袁静[1] 

机构地区：[1]东南大学医学院神经生物学研究所,南京210009 [2]扬州大学医学院附属扬州洪泉医院神经内科,扬州225200

出　　处：《神经解剖学杂志》2011年第6期604-610,共7页Chinese Journal of Neuroanatomy

摘　　要：目的:探讨转录因子垂体同源盒家族因子3(Pitx3)和孤儿核受体相关因子1(Nurr1)在多巴胺(DA)能神经元终末分化中的表达特点。方法:采用免疫荧光方法检测体外培养的中脑源性神经干细胞(mNSCs)诱导分化后和大鼠胚胎发育至出生腹侧中脑Pitx3、Nurr1和酪氨酸羟化酶(TH)的表达。结果:(1)体外培养的mNSCs诱导分化48 h的Map-2阳性细胞不表达Pitx3、Nurr1和TH;分化7 d的TH阳性细胞均表达转录因子Pitx3和Nurr1;(2)在大鼠腹侧中脑黑质(SN)、腹侧被盖区(VTA)和中缝背核(DRN)可见大量TH与Pitx3(或Nurr1)共表达的神经元;(3)Pitx3和Nurr1阳性细胞主要分布于E16.5、P0大鼠SN、VTA和DRN区,其中Pitx3阳性细胞还少量分布于腹侧中脑非DA能神经元区域,Nurr1阳性细胞在腹侧中脑分布范围较Pitx3更广泛。结论:转录因子Pitx3、Nurr1在中脑DA能神经元的终末分化和生存维持中起重要作用。Objective： To explore the characteristic of the transcription factors Pitx3 or nuclear-receptor related factorl（Nurr1） expression in the terminal differentiation of mesencephalic dopaminergic neurons.Methods： Immunofluorescence was used to observe the expression of Pitx3,Nurr1or tyrosin hydroxylase（TH） in the midbrain-derived neural stem cells（mNSCs） after differentiation in vitro and the ventral mesencephalon tissues from the stage of embryonic development to be born of rat.Results：（1）Map-2 positive cells did not express Pitx3,Nurr1 or TH in differentiating cells after 48 hours,but TH-positive cells all expressed Pitx3 or Nurr1 after 7 days in vitro;（2）The TH-positive cells were co-expressied with Pitx3（Nurr1） in substantia nigra（SN）,ventral tegmental area（VTA） and nucleus raphes dorsalis（DRN） of ventral mesencephalon of rats;（3）Pitx3 or Nurr1 positive cells mainly were distributed in SN,VTA and DRN of E16.5 and P0 rats,but less Pitx3-positive cells in non-DA neurons regions of ventral mesencephalon,the Nurr1-positive cells were distributed more extensive than Pitx3 in ventral mesencephalon.Conclusion： The transcription factor Pitx3 or Nurr1 might play an important role in the terminal differentiation and survival of mesencephalic dopaminergic neurons.

关 键 词：垂体同源盒家族因子3 孤儿核受体相关因子1 多巴胺能神经元 酪氨酸羟化酶 发育 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]